12-Hydroxy-5,8,10-heptadecatrienoic Acid Analysis Service
- Quantitative analysis of 12-HHT in biological samples.
- Profiling of metabolic pathways involved in 12-HHT metabolism.
- Analysis of the role of 12-HHT in various biological processes.
- Data interpretation and consultative support.
- Drug metabolism studies.
- Disease biomarker discovery.
- Therapeutic target identification.
- Animal tissue ≥ 100 mg
- Plant tissue ≥ 200 mg
- Serum/ Plasma ≥ 200 μL
- Urine ≥ 2 mL
12-Hydroxy-5,8,10-heptadecatrienoic acid (12-HHT) is a biologically active lipid that emerges as a byproduct of the cyclooxygenase (COX) pathway, specifically during thromboxane A2 (TXA2) synthesis. This metabolite features a hydroxyl group at position 12 and three conjugated double bonds at positions 5, 8, and 10, contributing to its unique chemical structure and bioactivity. Initially thought to be a secondary metabolite, 12-HHT has gained recognition for its significant roles in vascular biology, inflammation, and tissue repair.
One of the most notable biological effects of 12-HHT is its ability to increase prostacyclin (PGI2) production in the vascular system. Prostacyclin is a potent vasodilator and inhibitor of platelet aggregation, essential for maintaining vascular homeostasis. Research has shown that platelet-derived 12-HHT enhances prostacyclin production by acting on vascular cyclooxygenase, the enzyme responsible for prostaglandin synthesis. This interaction suggests that 12-HHT may play a regulatory role in balancing thromboxane and prostacyclin levels, which are crucial for controlling blood clotting and vascular tone. Additionally, 12-HHT has been identified as a ligand for the BLT2 receptor, influencing processes such as inflammation, wound healing, and chemotaxis.
Given its involvement in critical biological pathways, the accurate detection and quantification of 12-HHT are essential for understanding its role in vascular health, cardiovascular diseases, and inflammatory conditions. Furthermore, its ability to influence prostacyclin production makes it a potential target for therapeutic intervention in disorders related to platelet function, thrombosis, and vascular inflammation.
Figure 1. The Structure of 12-Hydroxy-5,8,10-heptadecatrienoic Acid
MtoZ Biolabs, an integrated Chromatography and Mass Spectrometry (MS) Services Provider, provides advanced proteomics, metabolomics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample consumption.
At MtoZ Biolabs, we offer comprehensive 12-Hydroxy-5,8,10-heptadecatrienoic acid analysis service that includes:
Analysis Workflow
Service Advantages
1. Advance Analysis Platform
MtoZ Biolabs established an advanced 12-Hydroxy-5,8,10-heptadecatrienoic acid analysis service platform, guaranteeing reliable, fast, and highly accurate analysis service.
2. One-Time-Charge
Our pricing is transparent, no hidden fees or additional costs.
3. High-Data-Quality
Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrate all 12-Hydroxy-5,8,10-heptadecatrienoic acid analysis service data providing clients with a comprehensive data report.
4. Fast Turnaround
The process from sample handling to report generation is efficient, shortening the analysis cycle.
Applications
Our 12-Hydroxy-5,8,10-heptadecatrienoic acid analysis service can be applied in various areas including:
Sample Submission Requirements
1. Sample Types
Serum, plasma, urine, tissues, and other biological samples. It is recommended to prepare at least 3 biological replicates for each sample type.
2. Sample Volume
3. Storage and Shipping
Samples should be stored at -80℃ and shipped with dry ice. Avoid repeated cycles of freezing and thawing
Note: Please provide details on sample collection and handling.
Deliverables
1. Comprehensive Experimental Details (Materials, Instruments, and Methods, etc.)
2. Relevant Liquid Chromatography and Mass Spectrometry Parameters
3. Detailed Information on 12-Hydroxy-5,8,10-heptadecatrienoic Acid
4. Raw Data
5. Custom Analysis Report
At MtoZ Biolabs, we are committed to providing high-quality, reliable, and efficient services to facilitate your research. Contact us today to discuss how we can assist with your 12-Hydroxy-5,8,10-heptadecatrienoic acid analysis needs.
How to order?
