10x TCR Sequencing Service
10x TCR Sequencing Service is an advanced high-throughput approach for characterizing T-cell receptor (TCR) repertoires at the single-cell level. T-cell receptors are essential components of the adaptive immune system, enabling T cells to recognize and respond to a vast array of antigens. These receptors, located on the surface of T cells, mediate antigen recognition by interacting with peptide-major histocompatibility complex (pMHC) molecules, initiating downstream immune responses.
A defining feature of TCRs is their immense diversity, known as the TCR repertoire diversity, which arises from V(D)J recombination and somatic hypermutation. This diversity ensures that the immune system can recognize virtually any foreign or mutated antigen, forming the basis of adaptive immunity. Characterizing the TCR repertoire provides invaluable insights into immune responses in infections, autoimmune disorders, and cancer immunotherapy. The 10x TCR Sequencing Service offers a powerful, high-resolution approach to unraveling TCR diversity at the single-cell level, facilitating breakthroughs in immunology and translational medicine.
Figure 1. Interaction Between an Antigen Presenting Cell (APC) and a T Cell, and V(D)J Recombination
Service at MtoZ Biolabs
MtoZ Biolabs provides 10x TCR Sequencing Service with a complete workflow, from single-cell isolation and library preparation to high-throughput sequencing and bioinformatics analysis. Leveraging the advanced platform, the 10x TCR Sequencing Service enables precise sequencing of TCR α (TRA) and β (TRB) chains, providing unparalleled insights into T-cell clonal diversity, antigen specificity, and immune response dynamics. 10x TCR sequencing captures the full complexity of the adaptive immune repertoire, by integrating single-cell barcoding, high-throughput amplification, and next-generation sequencing (NGS), offering high-resolution immune profiling crucial for immunological research, disease monitoring, and therapeutic development.
Figure 2. Schematic of Paired scRNA-Seq and TCR Sequencing Methods
Service Advantages
1. Cutting-Edge Platform: MtoZ Biolabs established an advanced 10x TCR Sequencing Service platform, ensuring high-resolution TCR profiling with exceptional accuracy and sensitivity.
2. Multi-Species Compatibility: The 10x TCR Sequencing Service supports a wide range of species, including humans and mice, enabling diverse immunological research.
3. Flexible Sample Acceptance: We accept various sample types, such as RNA, DNA, T cells, blood, and tissue, ensuring adaptability to different research needs.
4. Customizable Solutions: Tailored services are available to meet specific experimental requirements, ensuring optimal outcomes for each project.
5. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
Applications
1. Biomarker Discovery
By comparing TCR repertoires between healthy individuals and patients, the 10x TCR Sequencing Service helps identify unique T-cell signatures that serve as biomarkers for disease diagnosis, prognosis, and treatment monitoring.
2. Infectious Disease Research
The ability of 10x TCR sequencing to profile antigen-specific T-cell responses is instrumental in studying immune reactions to pathogens. This includes tracking T-cell clonotypes during infection progression and vaccine responses.
3. Immune Response to Malignancy
Understanding the diversity and clonality of tumor-infiltrating lymphocytes (TILs) is critical in oncology research. The service enables detailed analysis of TCR repertoires, helping to elucidate T-cell-mediated immune responses against tumors.
4. Cancer Immunotherapy
10x TCR Sequencing Service supports the development and evaluation of immunotherapeutic strategies, including adoptive T-cell therapies, cytokine-based interventions, and checkpoint inhibitors. It is also pivotal in monitoring T-cell responses to cancer vaccines, aiding in the design of more effective treatments.
Figure 3. Applications of 10x TCR Sequencing Service
Case Study
Unraveling Heterogeneous Antitumor Immunity with 10x TCR Sequencing Service
Antitumor immune responses vary significantly among individuals, influencing therapeutic outcomes in cancers such as head and neck squamous cell carcinoma (HNSCC). To investigate the underlying mechanisms, researchers developed a unique HNSCC mouse model and employed the 10x TCR Sequencing Service to analyze CD8 tumor-infiltrating lymphocytes (TILs). Despite genetic uniformity, ~25% of mice eradicated tumors without intervention, driven by CD8 T-cell activity. Single-cell TCR sequencing revealed distinct TCR clonotypes in regressors and progressors, with minimal overlap. Top clonotypes clustered in different activation states, highlighting the influence of the tumor microenvironment on T-cell responses. These findings underscore the highly individualized nature of antitumor immunity, emphasizing the role of 10x TCR Sequencing Service in decoding TCR diversity. This approach provides critical insights for developing personalized cancer immunotherapies.
FAQ
Q: What are the differences between TCR and BCR?
TCR (T-cell receptor) and BCR (B-cell receptor) are critical components of adaptive immunity with distinct roles. TCR is expressed on T cells and recognizes processed antigens presented by MHC molecules, requiring cell-cell interaction. BCR is expressed on B cells and directly binds unprocessed, native antigens in soluble or membrane-bound forms. Structurally, TCR comprises α and β (or γ and δ) chains, while BCR is an immunoglobulin with heavy and light chains. Functionally, TCR activation mediates cellular immune responses, whereas BCR activation leads to antibody production, bridging humoral immunity. Both are essential for immune system precision and diversity.
Deliverables
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Data Analysis and Preprocessing
4. Bioinformatics Analysis
5. Raw Data Files
6. The Detailed Information of TCR Sequencing
Our 10x TCR Sequencing Service is designed to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample consumption. Free project evaluation, welcome to learn more details.
How to order?
