10x Single Cell Sequencing Service
- High cell throughput: Capture from 1,000 to 10,000 cells in a single experiment, suitable for large-scale studies.
- Accurate molecular tagging: Unique Molecular Identifier (UMI) technology effectively distinguishes true signals from amplification noise.
- Multi-omics compatibility: Supports integration of transcriptomics, immune repertoire, ATAC-seq, and spatial transcriptomics data for comprehensive analysis.
- End-to-end quality control: Strict oversight from sample viability (>90%) to library concentration (≥2 nM).
- Customizable analysis: Detailed analysis including cell subpopulation annotations, pseudotime analyses, and cell interaction networks.
- Fast turnaround: From sample receipt to report delivery in just a few weeks.
- Immunology: Deepens understanding of immune cell types, functions, and interactions, advancing immunotherapy and vaccine development.
- Cancer Research: Resolves cellular heterogeneity in tumors, helping identify immune evasion mechanisms and treatment targets.
- Neuroscience: Provides data to support research into neurological disorders, promoting personalized treatments.
- Clinical & Personalized Medicine: Supports precision medicine by enabling personalized treatment plans for conditions like cancer and genetic diseases.
10x single cell sequencing is a high-throughput method that combines microfluidic sorting and molecular barcode tagging to analyze gene expression, epigenetics, and immune repertoire heterogeneity at the single-cell level. By attaching unique barcodes to each molecule within a cell, 10x single cell sequencing allows the precise capture of molecular information from thousands of individual cells, avoiding the masking of intercellular variability commonly seen in traditional bulk RNA sequencing.
Unlike bulk sequencing, 10x single cell sequencing uncovers the characteristics, dynamic changes, and interactions of cellular subpopulations, offering higher-resolution biological data. This approach is widely applied in cancer microenvironment studies, immune cell differentiation, and organ development research, providing fresh insights into the study of complex biological systems.
MtoZ Biolabs offers comprehensive 10x Single Cell Sequencing Service that are powered by cutting-edge technology platforms and extensive expertise. We assist clients at every stage of their projects, from experimental design to data analysis, ensuring optimal outcomes throughout the entire process.
Services at MtoZ Biolabs
Our 10x Single Cell Sequencing Service provides an advanced technology platform for the precise analysis of gene expression, epigenetics, and immune repertoire at the single-cell level. Comprehensive technical support is offered throughout the entire process, from initial consultation to data interpretation, ensuring methodological rigor and adherence to the highest standards to facilitate significant advancements in research.
1. Project Consultation and Solution Design
After the initial consultation, our expert team will provide a detailed research plan tailored to the project, covering experimental design, sample requirements, and the best technical approach.
2. Sample Preparation and Quality Control
We ensure high standards of sample preparation and quality control for various sample types, maximizing the success rate of subsequent experiments.
3. Library Construction and Sequencing
Using the 10x Genomics Chromium platform, we construct high-quality libraries and collect data through high-throughput sequencing, ensuring both data integrity and high throughput.
4. Data Analysis and Results Interpretation
Our expert analysis team offers both standardized and tailored analysis services to help you extract meaningful biological insights from your data.
5. Report Writing and Follow-up Services
We provide detailed reports with data analysis results, graphical presentations, and recommendations, along with ongoing technical support.
Cai, C. C. et al. Biomed. Pharmacother. 2023.
Figure 1. The Workflow of 10x Single Cell Sequencing
Advantages
1. Technical Advantages
2. Service Advantages
Applications
10x Single Cell Sequencing Service show enormous potential across diverse fields:
Case Study
Case 1
The INVADEseq technology detailed in this study combines single cell RNA sequencing with 16S rRNA sequencing to simultaneously analyze the transcriptome of host cells and their bacterial communities at single-cell resolution, revealing how bacterial adhesion or invasion impacts host gene expression. This study leverages the 10x single cell sequencing platform to demonstrate its capability in multi-omics integration, offering high-resolution solutions for microbiome-host interaction research, applicable to infectious diseases and microbiome studies.
Niño, J. L.G. Nat. Protoc. 2023.
FAQ
Q1: Which species are suitable for 10x Single Cell Sequencing Service?
The 10x single cell sequencing platform uses beads with poly-dT structures that bind to the polyA tail of mRNA molecules, making it suitable for species with polyA-tailed mRNA. However, certain eukaryotes, like those with mitochondria or chloroplast genes that lack a polyA tail, may be undetectable. Additionally, species should have high-quality genome assembly and annotation for effective analysis.
Q2: Is single cell sequencing possible for plants?
Plant single-cell research faces unique challenges, such as the presence of cell walls that require protoplast preparation before single-cell suspension. This process can be complicated by enzyme digestion and osmotic pressure issues. Additionally, plant tissue tends to have lower cell heterogeneity, making clustering analysis more difficult, and some large plant cells may not be compatible with the 10x single cell sequencing platform.
MtoZ Biolabs' 10x Single Cell Sequencing Service offers advanced support to help you interpret gene expression and cellular functions at the single-cell level. Whether your research focuses on fundamental science, immunotherapy, or personalized medicine, we provide high-quality data and customized analysis solutions. Contact us for more information and support.
How to order?
