10x RNA Sequencing Service
- Immune System Profiling: Analyze immune cell diversity and function in health and disease.
- Cancer Research: Study tumor heterogeneity, the tumor microenvironment, and immune responses.
- Stem Cell Research: Investigate differentiation pathways and reprogramming processes.
- Developmental Biology: Explore gene expression changes during development and cell fate determination.
- Neurology: Examine neuronal diversity and gene expression in brain development and disease.
10x RNA Sequencing is a cutting-edge technique that enables the precise analysis of gene expression at the single-cell level, providing unprecedented insights into cellular heterogeneity and dynamic biological processes. Mapping genotypes to phenotypes is one of the fundamental challenges in biology and medicine, and transcriptome analysis is a key approach to addressing this challenge. However, while all cells share nearly identical genomes, their transcriptomes vary significantly, as each cell expresses only a subset of genes at any given time. Conventional bulk RNA sequencing, which averages signals across cell populations, often masks critical cell-to-cell variability, missing rare cell states and failing to capture dynamic gene expression patterns.
By leveraging the 10x Genomics Chromium platform, the 10x RNA Sequencing Service overcomes these limitations by enabling high-throughput transcriptomic profiling at the single-cell level. Through microfluidic partitioning and unique molecular barcoding, thousands of individual cells can be analyzed simultaneously, revealing cell type-specific expression, identifying rare subpopulations, and uncovering the stochastic nature of gene expression that influences cell fate decisions. This level of precision is crucial for studying cell differentiation, immune system regulation, tumor microenvironments, and neurological development, where cellular diversity plays a fundamental role in biological outcomes.
Service at MtoZ Biolabs
MtoZ Biolabs provides a comprehensive 10x RNA Sequencing Service, covering sample preparation, sequencing, and advanced bioinformatics analysis. Our expertise ensures high-quality, reproducible data, allowing researchers to explore gene expression dynamics at single-cell resolution leveraging 10x RNA Sequencing Service. With customized analysis pipelines, MtoZ Biolabs‘ 10x RNA Sequencing Service help researchers extract meaningful insights into cellular function, disease mechanisms, and therapeutic targets, advancing discoveries in immunology, oncology, developmental biology, and neuroscience. Free project evaluation, welcome to learn more details!
Figure 1. An Overview of the Single-Cell RNA-Sequencing Procedures
Service Advantages
1. Advanced Analysis Platform: MtoZ Biolabs established an advanced 10x RNA Sequencing Service platform, guaranteeing reliable, fast, and highly accurate analysis service.
2. High Cell Throughput: The 10x RNA Sequencing Service enables the analysis of thousands of individual cells in a single experiment, providing comprehensive insights into cellular heterogeneity and dynamic gene expression across large sample sizes.
3. High Coverage: With the ability to capture a broad range of gene expression, including lowly expressed genes and rare cell types, the 10x RNA Sequencing Service ensures high coverage of the transcriptome, allowing for more accurate and in-depth analysis.
4. Broad Cell Type Adaptability: The 10x RNA Sequencing Service is suitable for a wide variety of cell types, including immune cells, cancer cells, stem cells, neuronal cells, and more, enabling researchers to study complex tissues and heterogeneous populations with precision.
5. High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrates all 10x RNA Sequencing Service data, providing clients with a comprehensive data report.
6. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
Applications
The 10x RNA Sequencing Service is applicable in various research fields, including:
Case Study
Decoding Astrocyte Differentiation Using 10x RNA Sequencing Service
In a groundbreaking study on astrocyte development, researchers utilized 10x RNA sequencing technology to analyze gene expression dynamics in astrocytes derived from both human-induced pluripotent stem cells (hiPSCs) and mouse embryonic stem cells (mESCs). The study profiled over 56,000 human cells and 113,000 mouse cells across multiple timepoints during differentiation, identifying intermediate and precursor cell populations that contribute to glial fate specification. Through high-resolution transcriptomic analysis, the team uncovered key regulatory genes and mapped associations between chromatin accessibility and gene expression using multi-omic sequencing. These insights enabled the optimization of differentiation protocols and revealed how signaling pathways, such as BMP4/FGFs and CNTF, impact astrocyte development. The 10x RNA Sequencing Service provided unparalleled single-cell resolution, facilitating the discovery of molecular mechanisms driving astrocyte maturation. These findings are crucial for advancing neurodegeneration research and developing models to study glial gene expression changes in disease contexts.
Frazel, P. W. et al. Nat Neurosci. 2023.
Figure 2. scRNA-Seq of Human Glial Differentiation Uncovers Lineage-Specific Precursors and Transient States
Frazel, P. W. et al. Nat Neurosci. 2023.
Figure 3. scRNA-Seq of Mouse Glial Differentiation Uncovers Lineage-Specific Precursors and Transient States
FAQ
Q: What are the differences between scRNA-Seq and snRNA-Seq?
scRNA-Seq (single-cell RNA sequencing) analyzes the transcriptome of individual cells, using whole cells that include both the nucleus and cytoplasm. snRNA-Seq (single-nucleus RNA sequencing), on the other hand, focuses on isolated nuclei from cells, often used for tissues with challenging cell isolation, such as the brain. While both provide insights into gene expression at the single-cell level, snRNA-Seq avoids issues with cell membrane integrity, capturing nuclear RNA, including transcriptional activity linked to chromatin regulation.
Deliverables
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Data Analysis and Preprocessing
4. The Detailed Information of 10x RNA Sequencing Results
5. Bioinformatics Analysis
6. Raw Data Files
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