10x Genomics Whole Genome Sequencing Service

10x Genomics whole genome sequencing is an innovative sequencing method based on the Linked-Reads technology, which integrates the high-throughput advantages of short-read sequencing with long DNA fragment connection information to overcome the limitations of traditional sequencing in analyzing complex genomic regions. 10x Genomics whole genome sequencing employs molecular barcodes to label long DNA fragments and uses bioinformatics algorithms to reconstruct sequence data, significantly enhancing the accuracy of structural variation, repetitive regions, and haplotype analyses. It is widely applied in cancer genomics, genetic disease research, population genetics, and other fields, especially suited for in-depth genomic analysis of high-complexity samples.

 

The 10x Genomics Whole Genome Sequencing Service provides a comprehensive solution from sample extraction to data analysis, offering researchers and clinical professionals high-precision genomic data. MtoZ Biolabs leverages its leading technology platform to deliver comprehensive genomic solutions, advancing scientific research.

 

Services at MtoZ Biolabs

MtoZ Biolabs' 10x Genomics Whole Genome Sequencing Service offers a comprehensive, flexible solution for high-quality and in-depth genomic analysis, tailored to meet diverse research needs:

· Standard Whole Genome Sequencing: Ideal for routine genome assembly and variation screening, covering a wide range of species.

· High Depth Sequencing: Designed for the analysis of low-frequency mutations or high-heterogeneity samples, enhancing detection sensitivity and accuracy.

· Single-Cell Whole Genome Sequencing: Assesses genomic heterogeneity within cell populations, revealing dynamics of microevolution.

· Customized Analysis: Provides specialized analyses, such as structural variations, copy number variations, and haplotype analysis, tailored to specific research goals.

 

Analysis Workflow

Our 10x Genomics Whole Genome Sequencing Service employs state-of-the-art technology and equipment to ensure the efficient and accurate completion of each project:

1. Sample Reception and Preparation

Upon receiving the samples, we conduct thorough quality control to ensure compliance with sequencing requirements.

 

2. DNA Extraction and Library Construction

High-quality genomic DNA is extracted, and libraries are constructed using the 10x Genomics platform. Each library is uniquely tagged with molecular barcodes for identification and tracking.

 

3. Sequencing and Data Acquisition

Using high-throughput sequencing technology, we sequence the libraries to obtain high-quality, long-read genomic data.

 

4. Data Processing and Analysis

We perform data alignment, quality control, variant detection, and other analyses using advanced bioinformatics tools to provide a comprehensive interpretation.

 

5. Report Generation and Delivery

We deliver the final analysis results in a detailed report, including key data such as structural variations, SNPs, and copy number variations.

 

Sample Submission Suggestions

To ensure the smooth execution of the 10x Genomics Whole Genome Sequencing Service, clients are advised to submit their samples according to the following requirements:

• Sample Types: Blood, tissue, PBMCs, cells, and other biological samples.
• Sample Quantity and Quality: A minimum of 10 μg of high-quality DNA with high purity and integrity is recommended.
• Sample Storage and Transport: Samples should be stored at low temperatures and avoid repeated freeze-thaw cycles to ensure stability. Transport should follow standardized procedures to prevent loss.

 

For detailed sample submission guidelines, please consult our technical team.

 

Why Choose MtoZ Biolabs?

1. Cutting-Edge Technology

We utilize the 10x Genomics Chromium platform, coupled with high-throughput sequencing, to deliver the most advanced genomic data.

 

2. Experienced Team and Comprehensive Support

Our skilled technical team offers full support throughout the project, from sample preparation to data analysis, ensuring high efficiency and quality.

 

3. Customized Solutions

We provide tailored sequencing strategies based on your research goals, ensuring optimal outcomes.

 

4. High-Quality Data and Reports

We deliver accurate and comprehensive data analysis reports, supporting both research and clinical applications.

 

Case Study

Case 1: Application of 10x Genomics Whole Genome Sequencing in Gene Editing

In this study, the 10x Genomics Whole Genome Sequencing revealed large-scale structural variations in the genomes of CRISPR-Cas9 edited mouse embryonic stem cells, including chromosome deletions, insertions, and translocations. These complex variations, driven by microhomology-mediated repair, could lead to functional gene disruptions. Unlike traditional short-read sequencing, which struggles to detect such large variations, 10x Genomics' Linked Reads technology offers an effective solution for accurately detecting megabase-scale rearrangements. This method provides critical high-resolution data for evaluating off-target effects in gene editing and ensures the safety of CRISPR-based gene therapies.

 



Tsai, H. H. et al. Nat. Commun. 2023.

 

 

Case 2: Application of 10x Genomics Whole Genome Sequencing in Plant Genomics

Using the 10x Genomics Whole Genome sequencing, researchers assembled and annotated the genome of Xanthoceras sorbifolium Bunge, overcoming the challenges of its complex genome. This high-resolution assembly provides crucial genetic data, supporting future research in plant genomics, genetic improvement, and ecology. The study highlights the capability of 10x Genomics Whole Genome Sequencing Service in unraveling complex plant genomes and lays the groundwork for future plant genomics research.

 



 

Liang, Q. et al. GigaScience. 2019.

 

Deliverables

Clients will receive the following standardized deliverables:

1. Raw Sequencing Data: In FASTQ format, compliant with international standards.

2. Analysis Report: Key results, including variation profiles, functional annotations, and visualized charts.

3. Advanced Analysis: Custom data packages such as haplotype genotyping and structural variation maps.

4. Technical Documentation: SOPs and data analysis methods.

5. Expert Interpretation: Biological insights and recommendations for key findings.