10x Genomics Single Cell Sequencing Service

Single cell sequencing technology, as a pioneering tool in the life sciences, has dramatically transformed our understanding of cells and their functions. Traditional bulk cell analyses cannot reveal the diversity among individual cells. In contrast, single cell sequencing captures heterogeneity at the single-cell level, offering researchers unprecedented insights. The emergence of this technology not only provides new perspectives for research in oncology, immunology, neuroscience, and other disciplines, but also drives the progress of precision medicine and personalized therapy.

 

Among the world’s leading single-cell analysis platforms, 10x Genomics has established itself as the industry benchmark due to its high throughput, high sensitivity, and broad applicability. As a professional biotechnology service provider, MtoZ Biolabs leverages 10x Genomics’ advanced platform, supported by a team of experienced scientists, state-of-the-art laboratory facilities, and customized service solutions, to deliver high-quality 10x Genomics Single Cell Sequencing Service—empowering research breakthroughs and driving innovation in clinical applications.

 

Technical Principles

10x Genomics single cell sequencing employs a droplet-based microfluidic system to precisely isolate individual cells and ensure their reaction with reagents within separate droplets. Each droplet contains the necessary reagents for cell lysis, barcoding, reverse transcription, and molecular labeling, promoting rapid cell lysis and release of mRNA. During this process, mRNA is captured by uniquely barcoded primers and reverse-transcribed into cDNA. Each cDNA molecule carries a distinct barcode and a unique molecular identifier (UMI) to counteract amplification bias, providing accurate digital quantification of transcript abundance. These cDNA molecules are subsequently amplified and used for library construction, followed by high-throughput short-read sequencing on a standard Illumina platform to obtain gene expression data at single-cell resolution.

 

By overcoming the limitations of conventional bulk sequencing approaches, the 10x Genomics platform enables high-precision gene expression analyses at the single-cell level. While data quality can be influenced by RNA integrity and batch effects, 10x Genomics single cell sequencing employs innovative barcoding methods and UMI correction to mitigate these challenges, delivering high-quality data for in-depth exploration of precision medicine, immunology, and disease mechanisms.

 



Source: 10X Genomics

 

Technical Advantages

·  Chromium System: Uses microfluidic technology for efficient and stable single-cell capture, ensuring high-quality and reproducible results.

·  Unique Molecular Tags (Barcode and UMI): Accurately distinguish individual cells and molecular events, reducing technical artifacts and improving data reliability.

·  Flexible Experimental Design: Tailor workflows to specific research objectives and sample characteristics, maximizing the value of the resulting data.

 

Services at MtoZ Biolabs

MtoZ Biolabs offers a suite of 10x Genomics Single Cell sequencing Services designed to meet diverse research needs and help customers extract critical biological information from complex systems. These solutions include:

1. Single-Cell Transcriptome Sequencing (scRNA-seq): Provides a comprehensive overview of gene expression at the single-cell level, unveiling cell types, states, and functions.

2. Single-Cell Immune Repertoire Sequencing (scVDJ-seq): Enables precise analysis of T- and B-cell receptor diversity, supporting immunology research.

3. Single-Cell ATAC Sequencing (scATAC-seq): Investigates chromatin accessibility on a per-cell basis, revealing gene regulatory mechanisms.

4. Spatial Transcriptome Sequencing (Visium): Integrates spatial context to uncover transcriptional heterogeneity within tissue microenvironments.

 

Why Choose MtoZ Biolabs?

1. Advanced Analysis Platform: MtoZ Biolabs established an advanced 10x Genomics single cell sequencing platform, guaranteeing reliable, fast, and highly accurate analysis service.

2. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.

3. High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrate all single cell sequence data, providing clients with a comprehensive data report.

4. Customized Service: Tailored to the specific research needs of our clients, we offer flexible experimental design and personalized data analysis to ensure the achievement of research goals to the fullest extent.

 

Case Study

Case 1: Application of 10x Genomics Single Cell Sequencing in Acute Myeloid Leukemia Research

This study employed 10x Genomics single cell sequencing to analyze acute myeloid leukemia (AML) cells, successfully identifying specific genetic alterations that affected gene expression and cell function. These findings highlight the potential of this technology to advance research into the molecular underpinnings of cancer. Through the platform’s high-resolution analyses, the study provided critical data for precise AML diagnosis and personalized treatment strategies, demonstrating the power of 10x Genomics Single Cell Sequencing Service for mutation detection and genomic studies at the cellular level.

 



 

Petti, A. A. et al. Nat. Commun. 2019.

 

 

Case 2: Application of 10x Genomics Single Cell Sequencing in Post-Stroke Depression Research

In this investigation, 10x Genomics single cell sequencing was performed on vascular endothelial cells, microglia, and oligodendrocytes in the hippocampus of a rat model of post-stroke depression. The results revealed transcriptomic alterations in these cell populations, offering new insights into the molecular mechanisms underlying post-stroke depression and laying the groundwork for biomarker discovery and therapeutic target development. This example underscores the value of 10x Genomics Single Cell Sequencing Service in neuroscience and disease mechanism research.

 



Li, C. et al. Mol. Psychiatry. 2024.

 

FAQ

Q1: Why perform single cell sequencing research?

Even neighboring cells within the same tissue (e.g., brain or heart) can exhibit marked heterogeneity, a phenomenon that is especially pronounced in tumors, which comprise various mutated cell populations. Traditional bulk sequencing, which measures genetic material from mixed cell populations and their microenvironment, may mask crucial details due to cellular diversity. 10x Genomics Single Cell Sequencing Service addresses this issue by significantly enhancing resolution at the single-cell level, enabling more precise insights into genetic information and providing critical perspectives for studies of development and disease.

 

The advent of single cell sequencing heralds the “individualization” era of cellular research. As a dedicated practitioner of 10x Genomics single cell sequencing technology, MtoZ Biolabs delivers reliable technical support to global research and clinical teams. Whether you are investigating disease mechanisms, developing novel therapies, or charting the blueprint of life, we can tailor an optimal solution for your needs. For further information, please contact us for a free consultation!