10x Genomics Sequencing Service
- Characterizing immune cell infiltration patterns and identifying immune therapy biomarkers.
- Investigating the transcriptional regulation networks of tumor stem cells and drug-resistant subpopulations.
- Mapping cell fate decisions during organ development.
- Tracking stem cell differentiation and functional integration after transplantation.
- Identifying brain-region-specific neuronal subtypes and synaptic plasticity mechanisms.
- Mapping the spatial spread of abnormal protein aggregation in neurodegenerative diseases.
- Investigating pathogen-specific T-cell clone expansion.
- Screening broad-spectrum neutralizing antibodies and optimizing their affinity maturation pathways.
10x Genomics sequencing enables high-throughput analysis of the transcriptome, epigenome, and immune repertoire of tens of thousands of individual cells through microfluidic sorting and molecular barcode labeling, effectively overcoming the limitations of traditional population sequencing that averages out individual cell data. In fields such as tumor microenvironment studies, immune cell dynamics, and neural circuit analysis, single-cell resolution allows for the precise identification of rare cell subpopulations, the exploration of cell-to-cell interactions, and the unraveling of functional evolution pathways, offering novel insights into complex biological mechanisms.
MtoZ Biolabs provides 10x Genomics Sequencing Service utilizing advanced technological platforms and extensive research expertise. We offer a range of solutions, including single-cell sorting, multi-omics integration, and spatial transcriptomics analysis, aimed at helping research and clinical teams address data challenges and support the advancement and application of scientific discoveries.
Technical Principles
The 10x Genomics sequencing technology is based on its unique single-cell capture and barcode system, enabling the acquisition of full genomic data for each cell in a single sequencing run. Specifically, the technology associates each individual cell with a distinct barcode, uses microfluidic chip technology to independently amplify each cell, and employs next-generation high-throughput sequencing to precisely sequence the amplification products.
Source: 10X Genomics
Services at MtoZ Biolabs
At MtoZ Biolabs, we provide a comprehensive range of 10x Genomics Sequencing Service that include everything from sample preparation to data analysis. This ensures that each study receives reliable, high-quality data support. Our services include three core modules:
1. Single-cell multi-omics sequencing: including single-cell RNA sequencing (scRNA-seq), single-cell ATAC-seq, and multi-omics integrated analysis (CITE-seq, Multiome).
2. Spatial transcriptomics sequencing: examining gene expression distribution within tissue and linking it to morphological and molecular features.
3. Immune receptor repertoire and antigen screening: tracking T/B cell receptor diversity and identifying pathogen-specific antibody sequences.
4. Customized integration solutions: supporting the coupling of single-cell and spatial omics data, or joint modeling with whole-genome sequencing analysis.
Service Advantages
Our 10x Genomics Sequencing Service offers numerous advantages:
· Single-cell resolution: uncovers rare subpopulations and transitional cells within broader cell populations, avoiding the averaging bias of bulk sequencing.
· Spatial context preservation: maintains the tissue's in-situ spatial coordinates, directly correlating gene expression with pathological features.
· Ultra-high throughput: processes tens of thousands to millions of cells in a single experiment, suitable for large-scale cohort studies.
· Clinical translational compatibility: compatible with FFPE samples, biopsy tissues, and other precious clinical specimens, facilitating the translation of basic research into clinical diagnostics.
Applications
10x Genomics sequencing technology is widely applicable, spanning numerous fields from basic biology to clinical research. Specific applications include:
1. Tumor Microenvironment Studies
2. Developmental and Regenerative Medicine
3. Neuroscience and Disease
4. Infection and Immune Response
Case Study
Case 1: 10x Genomics Sequencing in Staphylococcus aureus Sepsis Immunology Research
In this study, 10x Genomics sequencing was used on circulating plasma cells from patients with Staphylococcus aureus sepsis. By analyzing peripheral blood from 17 patients, over 400 plasma cells were identified, along with more than 200 unique immunoglobulin sequences. The research discovered four novel monoclonal antibodies, three of which cross-reacted with epidermal staphylococcus. This high-resolution single-cell RNA sequencing enabled in-depth analysis of plasma cells’ role in sepsis and provided key data for antibody development, showcasing the potential of 10x Genomics Sequencing Service in immunology and drug discovery.
Kerkman, P. K. et al. J. Immunol. 2024.
Case 2: 10x Genomics Sequencing in C9-ALS/FTD Research
This study used 10x Genomics sequencing on brain organoids from C9-ALS/FTD patients to uncover neurodevelopmental changes due to the C9ORF72 gene mutation. By analyzing blood samples from 17 patients, researchers identified over 400 plasma cells, revealing their diversity in immune responses and successfully screening four novel monoclonal antibodies. The 10x Genomics platform allowed precise analysis of gene expression dynamics and cell-type transitions in early-stage C9-ALS/FTD, demonstrating the platform’s utility in neurodegenerative disease research and offering potential insights into disease mechanisms and targeted therapeutic strategies.
van der Geest, A. T. et al. Acta Neuropathol. Commun. 2024.
FAQ
Q1: How many cells should be collected for each sample?
We recommend at least 1000 cells per sample for optimal representation of single-cell data. A lower cell count may compromise data quality, especially when analyzing rare subpopulations. If your study requires higher resolution, the cell count can be adjusted accordingly. MtoZ Biolabs provides tailored recommendations based on your specific research needs.
Q2: Does sample quality impact sequencing results?
We have optimized our protocols for low-quality or limited samples (e.g., biopsy or liquid biopsy) to minimize the impact of sample degradation. For such samples, MtoZ Biolabs offers pre-experiment evaluation to ensure data reliability, even for small or degraded samples, ensuring high-quality sequencing results.
At MtoZ Biolabs, we offer high-quality 10x Genomics Sequencing Service to help you efficiently and accurately analyze complex biological samples. With our cutting-edge technology, extensive industry experience, and superior customer support, we provide comprehensive assistance for your research. If you're looking for a reliable partner to deliver precise, efficient genomic research solutions, MtoZ Biolabs is your ideal choice.
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