1-methylpseudouridine Analysis Service
- High Sensitivity: State-of-the-art mass spectrometry technologies ensure high sensitivity and accuracy.
- Expertise: A team of experienced scientists ensures high-quality data and insightful analysis.
- Comprehensive Report: Provides a detailed report, making it easy to integrate the results into your research.
- Biomedical research
- Drug development
- Biomarker discovery
- Agricultural research
- Animal tissue ≥ 100 mg
- Plant tissue ≥ 200 mg
- Serum/ Plasma ≥ 200 μL
- Urine ≥ 2 mL
1-Methylpseudouridine (m1Ψ) is a chemically modified nucleoside that plays a critical role in RNA-based therapeutics, including mRNA vaccines. Structurally, m1Ψ is a derivative of pseudouridine, in which the nitrogen atom at position 1 of the pseudouridine ring is methylated. This methylation alters the chemical properties of the nucleoside, enhancing the stability and translation efficiency of synthetic mRNA while reducing its immunogenicity compared to unmodified uridine. These properties make m1Ψ an essential component in the development of advanced mRNA technologies, particularly in vaccines and therapeutics aimed at treating infectious diseases and various cancers.
Figure 1. The Structure of 1-methylpseudouridine
Recent research on 1-methylpseudouridine has focused on understanding its impact on RNA secondary structure, its role in improving mRNA stability, and its potential to reduce innate immune responses. It has been shown that the incorporation of m1Ψ into mRNA increases the efficiency of protein expression, contributing to the enhanced efficacy of mRNA-based vaccines. The modification helps evade immune system detection, thus reducing the activation of pattern recognition receptors that would otherwise limit the therapeutic effect of unmodified RNA.
The accurate and sensitive analysis of 1-methylpseudouridine is essential for quality control and the development of novel RNA-based therapies. With our state-of-the-art mass spectrometry platform and in-depth expertise in metabolomics, we can offer comprehensive 1-methylpseudouridine analysis service. We help our clients decipher the complex metabolome, uncovering the roles, and impact of 1-methylpseudouridine in their studies.
Analysis Workflow
Service Advantages
Our 1-methylpseudouridine analysis service offers:
Applications
Our service is widely applicable in various fields including but not limited to:
Sample Submission Requirements
1. Sample Types
Serum, plasma, urine, tissues, and other biological samples. It is recommended to prepare at least 3 biological replicates for each sample type.
2. Sample Volume
3. Storage and Shipping
Samples should be stored at -80℃ and shipped with dry ice. Avoid repeated cycles of freezing and thawing
Note: Please provide details on sample collection and handling.
Deliverables
1. Experimental Procedures
2. Relevant Liquid Chromatography and Mass Spectrometry Parameters
3. Detailed Information on 1-methylpseudouridine
4. Raw Data
5. Custom Analysis Report
For more information about our 1-methylpseudouridine analysis service or any other services at MtoZ Biolabs, please feel free to contact us. We are committed to providing solutions tailored to your specific research needs.
How to order?
