Validation Service of Cell Line Expression Products

Stable cell lines are crucial for applications in recombinant protein and antibody production, drug screening, and gene function research. They can grow continuously, stably carry genetic modifications or express transgenes, and maintain consistent expression levels. Industrial production of recombinant proteins employs two main methods: transient transfection and stable transfection. Transient transfection is used in early drug development to produce small amounts of protein for activity or animal testing. In contrast, stable cell line construction is essential for the industrial production of therapeutic proteins, ensuring consistent product quality across different times, locations, and batches.

 

However, after multiple generations of cultivation, cell lines may exhibit genetic instability. Verification of cell line expression products is primarily conducted through N-terminal sequencing. Edman degradation sequencing is a well-established technique that labels and cleaves N-terminal amino acids, revealing the protein's N-terminal sequence without disrupting other peptide bonds. This method is highly effective in verifying the accuracy of recombinant protein N-terminal sequences. Proper processing of N-terminal methionine and signal peptides during cell line establishment and fermentation is critical, as these steps directly impact protein function and stability.

 

Thus, verifying the correct processing of N-terminal methionine and signal peptides in protein products during cell line establishment and fermentation is crucial. MtoZ Biolabs offers advanced Edman sequencing systems and an experienced technical team to provide high-quality N-terminal sequencing services. Our Edman degradation method accurately determines sequences of up to 60-70 amino acids at the N-terminus of proteins and distinguishes highly similar amino acids such as I/L and Q/K, ensuring the stable and reliable quality of recombinant proteins.

 

Service Advantages

1. High Sensitivity 

Our technology offers high sensitivity in detecting low levels of PTH-AA.

 

2. Detailed N-terminal Sequence Analysis

Our technology is capable of analyzing sequences of 60-70 amino acids at the N-terminus, ensuring comprehensive coverage.

 

3. Accurate Amino Acid Differentiation

We can accurately distinguishe highly similar amino acids such as I/L and Q/K, ensuring the accuracy of sequence data.

 

Applications

1. Detection of Protein Expression Levels

By measuring the expression levels of target proteins in cell lines, the efficiency of the expression system and the yield of the product can be evaluated.

 

2. Protein Purity and Integrity

Validating the purity and integrity of the protein expressed by the cell line ensures there are no impurities or degradation products during production, guaranteeing product quality.

 

3. Post-translational Modification Analysis

Detecting and identifying post-translational modifications (such as glycosylation, phosphorylation, etc.) of proteins expressed by the cell line helps understand the impact of modifications on protein function and activity.

 

Deliverables

1. Experimental Procedures

2. Relevant Mass Spectrometry Parameters

3. Detailed Information on Validation of Cell Line Expression Products

4. Mass Spectrometry Images

5. Raw Data