Uracil-5-Carboxylic Acid Analysis Service
- Identification of uracil-5-carboxylic acid metabolites
- Quantitative analysis of uracil-5-carboxylic acid concentration
- Providing insights for therapeutic interventions
- Animal tissue ≥ 100 mg
- Plant tissue ≥ 200 mg
- Serum/ Plasma ≥ 200 μL
- Urine ≥ 2 mL
Uracil-5-carboxylic acid is a modified form of uracil, a pyrimidine nucleobase found in RNA. This derivative is characterized by a carboxylic acid group attached to the fifth carbon of the uracil ring. Its molecular formula is C₅H₄N₂O₄, with a molecular weight of 156.10 g/mol. Due to its polar nature, uracil-5-carboxylic acid can engage in a range of biochemical interactions, particularly involving nucleic acids and cellular signaling pathways. Elevated levels of uracil-5-carboxylic acid have been associated with oxidative stress and DNA damage, conditions commonly linked to the production of reactive oxygen species (ROS). Its accumulation may serve as a potential biomarker for oxidative damage to nucleic acids, and has been observed in certain pathological conditions, such as cancer, neurodegenerative diseases, and aging. The compound's presence indicates ongoing cellular stress or an impairment in DNA repair mechanisms.
The quantification of uracil-5-carboxylic acid in biological samples is generally achieved using mass spectrometry-based techniques like liquid chromatography-tandem mass spectrometry (LC-MS/MS). These methods provide high sensitivity and specificity, allowing for the detection and measurement of uracil-5-carboxylic acid even at low concentrations. Such analyses are crucial for investigating its physiological and pathological roles, monitoring its levels across various disease states, and assessing the impact of therapeutic interventions targeting oxidative stress and nucleic acid repair processes.
Figure 1. The Structure of Uracil-5-Carboxylic Acid
Uracil-5-carboxylic acid is a uracil derivative and is often used as an indicator in the study of nucleic acid metabolism. MtoZ Biolabs provides a state-of-the-art Uracil-5-Carboxylic Acid Analysis Service, powered by our sophisticated liquid chromatography-mass spectrometry (LC-MS) platform and professional metabolomics team.
Analysis Workflow
Service Advantages
1. Advanced Technological Platform
We use industry-leading mass spectrometry equipment and analysis techniques to ensure high precision and reliability of the analysis results.
2. Extensive Experimental Experience
With years of experience in experimental operations and data processing, we can handle various complex biological samples.
3. Rapid Data Delivery
Ensure quick sample processing and timely delivery of research results to facilitate scientific progress.
Applications
Sample Submission Requirements
1. Sample Types
Serum, plasma, urine, tissues and other biological samples.
At least 3 replicates for each sample type are required.
2. Sample Volume
3. Sample Preservation
Samples should be stored at -80°C to maintain stability.
Note: Detailed instructions on sample collection and handling will be provided.
Deliverables
1. Experimental Procedures
2. Relevant Liquid Chromatography and Mass Spectrometry Parameters
3. Detailed Information on Uracil-5-Carboxylic Acid
4. Raw Data
5. Custom Analysis Report
At MtoZ Biolabs, we strive to expedite your research and product development processes by supplying robust and accurate results. Through our uracil-5-carboxylic acid analysis service, we aim to provide comprehensive insights into nucleic acid metabolism.
How to order?
