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    Ubiquitinated Protein Analysis Service

      Ubiquitination involves the covalent attachment of ubiquitin molecules to target proteins, following these steps: (1) Activation: Ubiquitin-activating enzyme (E1) activates ubiquitin via ATP hydrolysis; (2) Conjugation: Ubiquitin-conjugating enzyme; (E2) transfers the activated ubiquitin to itself; (3) Ligation: Ubiquitin ligase (E3) identifies specific substrate proteins and attaches ubiquitin to the lysine residues of these proteins. This modification tags proteins for degradation, regulates their activity, alters their cellular localization, or affects their protein interactions. Ubiquitination is crucial in cell cycle regulation, signal transduction, and DNA repair. Abnormal ubiquitination is linked to diseases like cancer and neurodegenerative disorders, making its study significant for scientific and clinical advancements.

       

      MtoZ Biolabs offers comprehensive ubiquitinated protein analysis service, leveraging advanced mass spectrometry to elucidate the formation, function, and biological roles of protein ubiquitination. Our expert team and cutting-edge equipment ensure high-quality results, supporting your research endeavors effectively. For more information on our ubiquitinated protein analysis service or any inquiries, please contact us. We look forward to collaborating with you and advancing scientific research together.

       

      Analysis Workflow

      1. Sample Preparation

      Extracting and digesting proteins from cells or tissues.

       

      2. Enrichment of Ubiquitinated Peptides

      Utilizing anti-ubiquitin antibodies or affinity tags for peptide enrichment.

       

      3. Mass Spectrometry Analysis

      Performing LC-MS/MS to separate and identify enriched peptides.

       

      4. Data Analysis

      Identifying ubiquitination sites and patterns using databases and bioinformatics tools.

       

      Service Advantages

      1. High Sensitivity and Specificity

       Advanced mass spectrometry ensures efficient peptide enrichment and accurate identification.

       

      2. Professional Team

      Our experienced team provides comprehensive services from sample preparation to data analysis.

       

      3. Customized Services

      We offer tailored solutions and technical support to meet specific client needs.

       

      4. Fast Delivery

      We provide efficient processes and data analysis for timely, reliable results.

       

      Applications

      1. Cancer Research

      Investigating ubiquitination pathway abnormalities in tumors to identify therapeutic targets.

       

      2. Neuroscience

      Studying ubiquitination's role in neuronal function and neurodegenerative diseases.

       

      3. Cell Biology

      Understanding ubiquitination's regulatory mechanisms in biological processes like the cell cycle and signaling.

       

      4. Drug Development

      Assessing drug effects on protein ubiquitination and identifying new drug targets.

       

      Deliverables

      1. Experimental Procedures

      2. Relevant Mass Spectrometry Parameters

      3. Detailed Information on Ubiquitinated Protein Analysis

      4. Mass Spectrometry Images

      5. Raw Data

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