TMT Labeling Mass Spectrometry Service
- Sample Labeling: Extract proteins, digest into peptides, and label them with TMT reagents.
- Mixing and Separation: Mix labeled peptides in equal ratios and fractionate using liquid chromatography.
- Mass Spectrometry Detection: Perform high-resolution detection to capture peptide and reporter ion signals.
- Data Analysis: Identify and quantify proteins through database searches and comprehensive data analysis.
- Uncover protein expression differences in healthy vs. diseased samples to identify critical pathways.
- Explore drug-induced protein expression changes to understand therapeutic mechanisms.
TMT labeling mass spectrometry is an advanced, high-throughput protein quantification technique, widely used in proteomics, particularly for accurately analyzing differential protein expression in complex samples. This method uses stable isotope labeling to simultaneously quantify multiple samples, enabling the parallel analysis of up to 18 samples. This capability significantly enhances experimental throughput and analysis efficiency. TMT labeling mass spectrometry is suitable for a wide range of biological samples, including cells, tissues, and body fluids, and has broad applications in biomarker discovery, disease mechanism studies, and drug development.
MtoZ Biolabs offers high-quality TMT labeling mass spectrometry service, leveraging the Thermo Scientific Orbitrap Fusion Lumos mass spectrometer and Nano-LC system. These cutting-edge technologies ensure accurate and reliable quantitative results, driving innovation and breakthroughs in various research fields.
Technical Principles
TMT labeling mass spectrometry utilizes stable isotope labeling reagents to chemically tag peptides, ensuring that peptides from different samples have the same mass-to-charge ratio in the first stage of mass spectrometry (MS1). In the second stage (MS2), the samples are distinguished by characteristic reporter ions, enabling precise quantification. The method supports a variety of multiplexing options, including 2-plex, 6-plex, 10-plex, 16-plex, and 18-plex, making it highly adaptable to different research needs requiring high-throughput analysis.
Analysis Workflow
MtoZ Biolabs' TMT labeling mass spectrometry service adheres to a rigorous and scientifically validated workflow. Each step, from sample preparation to data analysis, is meticulously controlled to ensure reliable and reproducible results.
Figure 1. Workflow for Relative Quantification Using 8 plex TMT
Why Choose MtoZ Biolabs?
1. Advanced Analysis Platform: MtoZ Biolabs established an advanced TMT labeling mass spectrometry platform, guaranteeing reliable, fast, and highly accurate analysis service.
2. High-Throughput Quantification: Supports comparative analysis of up to 18 samples, boosting experimental throughput.
3. Enhanced Sensitivity: Detects low-abundance proteins with precision across a broad dynamic range.
4. Wide Applicability: Suitable for analyzing cells, tissues, body fluids, and other biological materials.
5. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
Sample Submission Suggestions
For more sample details, please consult our technical team.
Applications
1. Disease Mechanism Research
2. Biomarker Discovery
Detect and validate differentially expressed proteins for disease diagnosis or prognosis.
3. Drug Development
Identify novel drug targets and monitor protein expression dynamics during treatment.
4. Systems Biology
Construct proteomic interaction networks to analyze complex biological systems.
5. Agriculture and Environmental Science
Evaluate stress responses in plants and assess pollutant impacts on organisms.
Case Study
1. Quantitative analysis of protein expression changes in GBM cells using TMT labeling mass spectrometry reveals the role of TRIM22 in regulating tumor proliferation and sensitivity to TMZ.
Fei, X. et al. Mol Ther Oncolytics. 2022.
FAQ
Q1: How can TMT labeling efficiency be optimized to reduce cross-labeling and signal interference?
Achieving optimal TMT labeling efficiency is crucial for experimental success. MtoZ Biolabs' TMT labeling mass spectrometry service ensures this by strictly controlling labeling reaction parameters, including pH, temperature, and reaction time. High-purity TMT reagents are employed to maximize labeling completeness. To further minimize cross-labeling and signal interference, excess labeling reagents are used, followed by thorough purification to remove unreacted materials. Labeling efficiency is rigorously assessed post-reaction to confirm complete labeling of peptides. With these refined protocols, MtoZ Biolabs delivers precise, high-quality TMT labeling services, ensuring reliable quantitative data and robust experimental outcomes.
What Could be Included in the Report?
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment
4. Data Analysis, Preprocessing, and Estimation
5. Bioinformatics Analysis
6. Raw Data Files
MtoZ Biolabs, an integrated Chromatography and Mass Spectrometry (MS) Services Provider, provides advanced proteomics, metabolomics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample consumption. Free project evaluation, welcome to learn more details!
How to order?
