SP3 Proteomics Service

SP3 proteomics (single-pot solid-phase-enhanced sample preparation) technology is an innovative and efficient sample preparation method that has rapidly become a cornerstone in proteomic analysis due to its streamlined operational workflow and superior performance. Compared to traditional FASP (filter-aided sample preparation) and in-solution digestion methods, SP3 proteomics employs functionalized magnetic beads to achieve sample cleanup, protein enrichment, and enzymatic digestion within a single operational step. This approach significantly enhances the efficiency and accuracy of sample preparation. When integrated with mass spectrometry technology, SP3 further optimizes proteomic analyses, making it an indispensable tool for high-throughput proteomic research.

 



Hughes, C. S. et al. Mol Syst Biol. 2014.

Figure 1. Schematic of the SP3 Proteomics Workflow in a Single Tube

 

MtoZ Biolabs offers advanced SP3 Proteomics Service to clients. By integrating our service with state-of-the-art mass spectrometry platforms, we provide efficient, precise, and scalable proteomic analyses. Whether your research objectives involve basic scientific exploration, elucidating disease mechanisms, or analyzing clinical samples, MtoZ Biolabs delivers professional technical support and service to facilitate breakthrough advancements in proteomic research.

 

Technical Advantages

• High Efficiency and Simplicity: Consolidated into a single operational step, reducing sample loss and minimizing processing time.
• High Throughput: Optimized for large-scale sample processing, ensuring high data throughput and reproducibility.
• Broad Compatibility: Suitable for various sample types, including blood, cells, tissues, and other complex biological samples.
• Effective Interference Removal: Efficiently eliminates impurities, enhancing the sensitivity and precision of subsequent analyses.
• Enhanced Digestion Efficiency: Improves enzymatic digestion through bead-surface interactions, ensuring high-quality data output.

 

Analysis Workflow

The SP3 proteomics service offers a streamlined, high-efficiency workflow that ensures optimal sample preparation for proteomics analysis. Each step is carefully designed to enhance sample purity and minimize loss, providing reliable and reproducible results, even for large-scale studies.

1. Sample Collection and Preprocessing

Conduct preliminary processing of biological samples to remove interfering substances and prepare samples compatible with SP3 protocols.

 

2. Magnetic Bead Binding

Introduce functionalized magnetic beads to the sample. The affinity ligands on the beads selectively bind target proteins, effectively separating them from impurities and interfering substances.

 

3. Sample Cleanup

Apply high-concentration organic solvents (e.g., ethanol or acetonitrile) to remove contaminants such as lipids and small molecules, ensuring the purity of protein samples.

 

4. Enzymatic Digestion and Enrichment

Add digestive enzymes (e.g., trypsin) to facilitate protein digestion under optimized conditions, followed by peptide enrichment using magnetic beads.

 

5. Mass Spectrometry Analysis Preparation

Post-digestion, perform appropriate washing and concentration steps to prepare the sample for mass spectrometry analysis.

 



Applications

1. Basic Research: Investigate protein expression, post-translational modifications, and functions to address fundamental questions in life sciences.

2. Disease Mechanism Studies: Identify alterations in disease-associated proteins to advance the understanding of disease pathogenesis.

3. Clinical Sample Analysis: Process clinical specimens for early disease diagnosis, biomarker discovery, and comprehensive clinical analyses.

4. Drug Development: Utilize SP3 technology for proteomic analyses in drug screening and elucidating mechanisms of action.

 

Case Study

Case 1: SP3 Proteomics Facilitates Biomarker Discovery for Primary Sclerosing Cholangitis (PSC)

This study integrated SP3 proteomics with high-precision mass spectrometry to perform a comprehensive analysis of the bile proteome. The approach successfully identified multiple differential proteins associated with PSC. The efficient sample preparation provided by SP3 significantly enhanced protein recovery and data quality in complex bile samples, ensuring the sensitivity and reliability of mass spectrometry analyses.

 

 

Kan, M. et al. J Hepatobiliary Pancreat Sci. 2022.

 

Case 2: SP3 Proteomics Drives Breakthroughs in High-Throughput Proteomic Research

Utilizing SP3 sample preparation in conjunction with the latest Orbitrap Astral mass spectrometer, this study efficiently analyzed biological fluids, cells, and tissue samples, identifying over 10,000 proteins. The coverage of low-abundance proteins increased by nearly 30%, and tissue sample proteome coverage exceeded 80%. SP3 technology streamlined the sample processing workflow, maintained data reproducibility with relative standard deviation (RSD) below 5%, and demonstrated exceptional performance in handling complex samples and detecting low-abundance proteins. These results underscore the pivotal role of SP3 proteomics service in advancing proteomic research and precision medicine.

 

 

Hendricks, N. G. et al. J. Proteome Res. 2024.

 

Why Choose MtoZ Biolabs?

1. Advanced Analysis Platform: MtoZ Biolabs established an advanced SP3 proteomics analysis platform, guaranteeing reliable, fast, and highly accurate analysis service.

2. High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrate all proteomics analysis data, providing clients with a comprehensive data report.

3. Comprehensive Services: From sample preparation to data analysis, MtoZ Biolabs provides end-to-end services, enabling you to conduct your research efficiently and effectively.

4. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.

 

As a specialized proteomics service provider, MtoZ Biolabs leverages advanced SP3 technology to deliver efficient, precise, and scalable SP3 proteomics service tailored to your research needs. Regardless of the scientific challenges you encounter, our professional technical support and comprehensive services are designed to help you achieve significant breakthroughs in your proteomic research.