Single Cell Pathway Analysis Service
The Single Cell Pathway Analysis Service is designed to provide comprehensive insights into cellular processes by analyzing biological pathway activities at the single-cell level. The Single Cell Pathway Analysis Service enables the detailed exploration of complex interactions between genes, proteins, metabolites, and other molecular entities within individual cells. By studying cellular processes at this resolution, the Single Cell Pathway Analysis Service can uncover subtle variations in cellular behavior that are often masked in bulk analyses, offering a clearer understanding of underlying biological mechanisms. MtoZ Biolabs specializes in providing integrated Single Cell Pathway Analysis Service.
Using advanced technologies like single-cell RNA sequencing, proteomics, and metabolomics, the single cell pathway analysis identifies key pathways and regulatory networks that drive cellular behavior. By applying pathway enrichment analysis, we reveal active pathways that influence cellular functions and responses. The single cell pathway analysis approach is invaluable in areas such as disease research, drug development, stem cell studies, and personalized medicine, providing the precision needed to drive innovation and enhance therapeutic strategies.
Service Advantages
1. Comprehensive Multi-Omics Pathway Analysis
The Single Cell Pathway Analysis Service offers a complete pathway analysis across multiple omics layers, including transcriptomics, proteomics, and metabolomics. The service can also integrate these diverse datasets to provide a comprehensive pathway map, giving deeper insights into cellular functions and regulatory networks.
2. Uncovering Cell Heterogeneity
Unlike traditional bulk analysis, this service provides a detailed view of individual cells, allowing for the identification of cell-to-cell variations and better understanding of complex biological systems, such as tissue heterogeneity or disease progression.
3. One-Stop Solution
The Single Cell Pathway Analysis Service provides a one-stop solution, covering the entire analysis workflow from sample preparation, data acquisition, to pathway enrichment analysis and interpretation. This integrated approach streamlines the process and saves time for researchers.
4. Customizable Service
The Single Cell Pathway Analysis Service can be tailored to suit specific research goals, whether for cancer studies, immune response analysis, or metabolic disease research, allowing for a focused and highly relevant analysis based on the research objectives.
Applications
1. Disease Research
Analyze cellular pathway alterations in diseases such as cancer and autoimmune disorders, uncovering molecular mechanisms driving disease progression.
2. Drug Development
Assess how cells respond to therapeutic treatments, identifying target pathways and understanding drug efficacy at the single-cell level.
3. Stem Cell Research
Investigate stem cell differentiation, lineage development, and cellular reprogramming to better understand stem cell biology.
Case Study
1. Single Cell Pathway Analysis in Hepatocellular Carcinoma (HCC)
In this study, single-cell RNA sequencing (scRNA-seq) was used to analyze immune cells from peripheral blood, tumor tissues, and adjacent normal tissues of four HCC patients and two non-cancer controls. A total of 212,494 cells were profiled to identify distinct immune cell subtypes and enriched pathways related to HCC progression. The analysis revealed APOC1 overexpression in tumor-associated macrophages (TAMs) of HCC tissues, with inhibition of APOC1 promoting the transition from M2 to M1 macrophages via the ferroptosis pathway. This shift was shown to enhance anti-PD1 immunotherapy responses. The Single Cell Pathway Analysis Service enabled the identification of key immune pathways and cell subtype dynamics, providing insights into how immune microenvironments influence treatment efficacy. This service is vital for uncovering therapeutic targets and improving the design of personalized treatments in diseases like HCC.
Figure 1. Inhibition of APOC1 Reversed the M2 Phenotype to the M1 Phenotype via the Ferroptosis Pathway in TAMs from HCC
2. Single Cell Pathway Analysis in Prostate Cancer Bone Metastasis
In this study, single-cell RNA sequencing was employed to analyze disseminated tumor cells (DTCs) in prostate cancer (PCa) bone metastases. By building a bone metastasis model and performing multi-omics analysis, including transcriptomic, proteomic, and phosphoproteomic approaches, the research identified a unique cluster of myeloid-like tumor cells. These hybrid cells, formed through cell fusion between disseminated tumor cells and bone marrow cells, exhibited significant changes in pathways related to immune regulation, cell adhesion, and proliferation. The single cell pathway analysis provided insights into how these hybrid cells contribute to tumor progression, immune microenvironment changes, and resistance to treatment. In vivo experiments showed that hybrid cells had higher proliferation rates, enhanced metastatic potential, and resistance to docetaxel, while remaining sensitive to radiotherapy. These findings suggest that myeloid-like hybrid cells in the bone marrow could be a potential therapeutic target for PCa bone metastasis, highlighting the importance of Single Cell Pathway Analysis Service in understanding tumor biology and identifying novel treatments.
Figure 2. GO Enrichment Analysis of Up-Regulated Genes and Proteins in Tumor Hybrid Cells
Deliverables
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Data Analysis, Preprocessing, and Estimation
4. The Detailed Information of Single Cell Pathway Analysis
5. Raw Data Files
Our Single Cell Pathway Analysis Service is designed to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample consumption. Free project evaluation, welcome to learn more details.
How to order?
