SDS-PAGE Measures Protein Molecular Weight: How Is Migration Distance Measured
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Conduct SDS-PAGE using a protein standard mixture with known molecular weights.
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Measure the migration distances of these standard proteins and generate a plot of molecular weight as a function of migration distance. This plot is typically nonlinear and can be fitted using a log-log transformation.
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Determine the migration distance of the unknown protein.
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Use the standard curve to estimate the molecular weight of the unknown protein.
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is a widely used technique for determining protein molecular weight. Following SDS-PAGE, the molecular weight of proteins can be estimated by measuring their migration distance within the gel.
The migration distance of proteins can be determined using image analysis software (e.g., ImageJ) or a ruler by measuring the distance from the sample well to the protein band.
Estimating Protein Molecular Weight Using Migration Distance:
1. Constructing a Standard Curve
2. Estimating the Molecular Weight of Unknown Proteins
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