Resources

Proteomics Databases



Metabolomics Databases

• • Isolation, Purification, and Characterization of Protein

  The isolation, purification, and characterization of protein involve processing complex biological samples using a series of specialized techniques to extract, purify, and analyze protein molecules. Proteins play a fundamental role in cellular structure and function, and comprehensive protein studies are essential for understanding biological processes and their regulatory mechanisms. The systematic isolation, purification, and characterization of protein enable researchers to investigate its structur......

• • Enzyme Characterization Methods

  Enzyme characterization methods are essential for examining the physical, chemical, and biological properties of enzymes. These protein molecules catalyze biochemical reactions within organisms with remarkable efficiency and specificity. Characterizing enzymes enhances our understanding of biochemical pathways, aids in drug development, improves the application of industrial enzymes, and optimizes biological processes. In biomedicine, enzyme characterization methods help to reveal the mechanisms of di......

• • Enzyme Characterization Techniques

  Enzyme characterization techniques comprise a set of methods in biochemistry and molecular biology, specifically designed to explore the structural, functional, and kinetic properties of enzymes. These proteins catalyze essential biochemical reactions and are ubiquitous in living organisms, playing crucial roles in nearly all biological processes. Through various experimental methodologies and analytical tools, enzyme characterization techniques enable the precise assessment of enzyme activity, stabil......

• • De Novo Sequencing Using Mass Spectrometry

  De Novo sequencing using mass spectrometry is a pivotal technique for elucidating the amino acid sequences of proteins and peptides. By accurately measuring the mass-to-charge ratios of ionized molecules or molecular fragments, mass spectrometry provides detailed insights into their composition and structure. Typically, De Novo sequencing incorporates liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the efficient separation and analysis of complex biological samples. This methodology is e......

• • Edman Method of Protein Sequencing

  The Edman method of protein sequencing is based on the reaction of phenyl isothiocyanate (PITC) with the N-terminal amino acid of a polypeptide chain, forming a labeled derivative that is subsequently cleaved under acidic conditions. This step enables the sequential identification of amino acids, with one residue being determined per reaction cycle. By iterative cycles, the complete sequence of the polypeptide chain can be elucidated.   In biomedical research, determining protein sequences is crucial ......

• • Recombinant Protein Characterization

  Recombinant protein characterization involves a comprehensive evaluation of proteins produced via genetic recombination technologies, employing multiple analytical methodologies. The primary objective is to confirm that these proteins' structural, functional, and purity attributes align with specific application demands, ensuring the absence of impurities or deviations. As products of contemporary biotechnology, recombinant proteins find extensive applications across pharmaceuticals, agriculture, and ......

• • Polysaccharide Characterization

  Polysaccharide characterization involves a comprehensive analysis and measurement of their structures, compositions, and physicochemical properties. Polysaccharides, a type of complex biological macromolecules found extensively in nature, have diverse structures and functions and are widely utilized in industries such as food, pharmaceuticals, and materials. The primary goal of polysaccharide characterization is to elucidate their structural characteristics, including monosaccharide composition, linka......

• • Proximity Labeling Mass Spectrometry

  Proximity labeling mass spectrometry is a powerful technique for investigating protein interactions and their spatial distribution within cells. This method involves labeling adjacent biomolecules to facilitate their identification and quantification via mass spectrometry. By merging chemical labeling with high-resolution mass spectrometry, this approach enables the detection of protein-protein interactions, protein localization, and dynamic changes within cellular environments. The central step in pr......

• • Polyclonal Antibody Sequencing

  Polyclonal antibody sequencing is a technique for determining the amino acid or nucleic acid sequences of antibody molecules within polyclonal antibodies. As components of the immune system, antibodies exhibit significant diversity due to complex biological processes such as gene rearrangement and somatic hypermutation. Polyclonal antibody sequencing plays a crucial role in unraveling the complexity of antibody repertoires. Unlike traditional monoclonal antibody approaches, which offer advantages in s......

• • Protein-Protein Interactions Methods for Detection and Analysis

  To gain deeper insights into the complexity of protein-protein interactions, scientists have developed various protein-protein interactions methods for detection and analysis. These interactions are fundamental to complex signaling networks, metabolic pathways, and cellular functional modules. The methods for studying protein-protein interactions can be broadly categorized into in vitro and in vivo approaches.   In Vitro Protein-Protein Interactions Methods for Detection and Analysis In vitro methods ......