Resources

Proteomics Databases



Metabolomics Databases

• • How to Analyze Histone Butyrylation by LC-MS/MS?

  Histone modifications represent a fundamental aspect of epigenetic regulation. Among these, butyrylation, an emerging short-chain fatty acid-derived post-translational modification (PTM), has been increasingly recognized for its roles in gene regulation, metabolic homeostasis, and disease development. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) enables not only precise identification of modification sites but also quantitative comparison of butyrylation levels across different biological......

• • Antibody Peptide Mapping by LC-MS/MS: Workflow, Applications, and Quality Attributes

  A practical guide to antibody peptide mapping by LC-MS/MS, including digestion, peptide separation, sequence coverage, PTM identification, degradation monitoring, biosimilar comparability, and QC considerations.

• • What Is Label-Free Analysis? LFQ Principles, Workflow, Advantages, and Limitations

  A practical guide to label-free analysis and label-free quantification (LFQ), including MS1 intensity, spectral counting, LC-MS/MS workflow, normalization, missing values, batch effects, and comparison with labeling approaches.

• • Label Transfer in Protein Interaction Studies: Principle, Workflow, and Method Selection

  A practical guide to label transfer for protein interaction studies, including bait-prey labeling, activation, label transfer, MS detection, applications, controls, limitations, and method selection.

• • Top-Down Protein Sequencing Principle: Intact Protein MS, Fragmentation, and Proteoform Analysis

  A practical guide to top-down protein sequencing, including intact protein preparation, high-resolution MS, fragmentation, proteoform analysis, PTM preservation, applications, and limitations.

• • How Post-Translational Modifications Affect Protein Function?

  A practical guide to how post-translational modifications regulate protein activity, stability, localization, interactions, signaling, metabolism, disease mechanisms, and MS-based PTM analysis.

• • Glycan Profiling Methods vs Phosphoproteomics: Key Differences in Targets, Workflows, and Data

  A practical comparison of glycan profiling methods and phosphoproteomics, including analytical targets, sample preparation, LC-MS workflows, enrichment strategies, applications, limitations, and method selection.

• • Protein Analysis Mechanisms: How Extraction, Separation, Identification, and Quantification Work

  A practical guide to protein analysis mechanisms, including sample preparation, separation, LC-MS/MS identification, quantification, PTM analysis, structural methods, limitations, and method selection.

• • 4D Label-Free Quantitative Proteomics Workflow: From Sample Prep to Data Interpretation

  A practical guide to the 4D label-free quantitative proteomics workflow, including sample preparation, ion mobility, PASEF acquisition, data analysis, QC, and method limitations.

• • Protein Interaction Network Analysis for Disease Research and Target Discovery

  A practical guide to protein interaction network analysis, including disease mechanism research, drug target discovery, omics integration, method selection, and evidence-quality limits.