Resources

Proteomics Databases



Metabolomics Databases

• • Shotgun Quantitative Proteomics

  Proteomics, the large-scale study of proteins, examines protein expression, modifications, interactions, and functions through specific experimental designs and techniques. With advances in mass spectrometry, quantitative proteomics has become a pivotal research area. Specifically, shotgun quantitative proteomics—a novel biochemical analysis method—offers new perspectives on the complexities of biological systems due to its high efficiency, precision, and comprehensive nature.

• • The Ellipticity Value of the Circular Dichroism Spectrum Is Less Than 1

  Ellipticity in Circular Dichroism (CD Ellipticity) quantifies the differential absorption of left- and right-handed circularly polarized light by optically active molecules. This parameter is widely used in the study of secondary structures in proteins and nucleic acids. Definition of Ellipticity: The ellipticity (θ) is expressed in degrees and represents the extent of circular dichroism in an optically active sample. If a substance absorbs left- and right-handed circularly polarized light equally, then....

• • Hydrogen Deuterium Exchange Mass Spectrometry Results Analysis

  Hydrogen Deuterium Exchange Mass Spectrometry (HDX-MS) is a valuable technique for identifying key dynamic regions within biomolecules. By measuring the rate of hydrogen-deuterium exchange, HDX-MS provides insights into whether specific regions are exposed or interact with other molecular domains. Principle: The underlying principle of HDX-MS is the exchange reaction between hydrogen and deuterium, where a hydrogen atom in a biomolecule is substituted by a deuterium atom. This substitution is detectable....

• • How to Master Protein Identification for Precise Biomarker Discovery

  Protein identification technologies are essential for the discovery, validation, and application of biomarkers. Biomarkers are biomolecules that indicate biological process states, disease progression, or drug responses. Among them, proteins, due to their essential roles in biological processes, represent a biomarker class with significant potential for clinical translation. Precise biomarker analysis relies on efficient and accurate protein identification techniques to ensure that identified proteins......

• • Protein Identification: 12 Common Mistakes to Avoid

  Protein identification is a crucial step in proteomics research. By precisely analyzing and identifying proteins within a sample, scientists can elucidate the fundamental roles of proteins in cellular function, disease mechanisms, and drug development, among other areas of biological research. However, due to the technical complexity and sample variability, protein identification often presents significant challenges. In this article, we summarize 12 common mistakes encountered during protein .......

• • How to Accurately Identify Proteins? Key Methods and Experimental Strategies

  Accurate protein identification is an integral part of life science research, involving the precise recognition and analysis of proteins in biological systems. The core of accurately identify proteins lies in utilizing high-precision technologies to achieve efficient and accurate protein identification while optimizing experimental strategies to ensure data reliability and reproducibility. With the advancement of life science research and proteomics technologies, researchers continuously explore more ......

• • Comprehensive Guide to Protein Identification: Techniques, Applications, and Data Analysis

  Protein identification is an integral part of life sciences research, with broad applications in areas such as disease research, new drug development, biomarker screening, and synthetic biology. Accurate protein identification depends on the use of advanced technologies, systematic experimental design, and robust data analysis strategies. Researchers currently employ a variety of techniques, including mass spectrometry, immunoassays, amino acid sequencing, and bioinformatics tools, to address the dive......

• • Sample Preparation Before Protein Mass Spectrometry

  Prior to conducting proteomics mass spectrometry, several preparatory steps must be taken to ensure both the successful execution of the experiment and the accuracy of the results. These steps include the selection of an appropriate mass spectrometer, meticulous sample preparation, protein extraction and purification, enzymatic digestion and labeling, sample preparation for mass spectrometry, and the precise calibration of the mass spectrometer.

• • Capillary Electrophoresis Chromatography Data Analysis

  Capillary Electrophoresis (CE) is a rapid, efficient, and high-resolution technique widely used for the separation and quantification of diverse biomolecules. To effectively interpret electropherogram data, it is essential to understand certain foundational methodologies and strategies. Data Preparation and Preprocessing: Initially, raw electropherogram data is exported from the experimental apparatus, comprising time and signal intensity at each detection point. The preprocessing phase involves noise......

• • How to Analyze the Results of Phosphorylated Protein WB

  Western blot (WB) analysis of phosphorylated proteins involves the following key steps: 1. Verification of Band Presence and Clarity: The first step is to examine the electrophoresis membrane for the presence of distinct protein bands and assess their clarity. For phosphorylated proteins, particular attention should be given to the presence of multiple bands, as phosphorylation can alter electrophoretic mobility.