Resources

Proteomics Databases



Metabolomics Databases

• • ADA Detection: Sample Collection, Processing, and Analysis

  "Antidrug antibodies" (ADA) usually refers to antibodies produced by the immune system against certain drugs, particularly biologics. When patients receive certain biologic therapies, such as monoclonal antibody therapy, their immune system may produce these antibodies, which can affect the safety and efficacy of the drugs. ADA testing is performed to determine whether these antidrug antibodies are present in the patient's body.

• • Experimental Steps to Determine Protein Extinction Coefficient

  The extinction coefficient of a protein is usually related to its absorbance at a specific wavelength, such as 280 nm. This is mainly because certain amino acid residues in proteins, such as tryptophan, tyrosine, and cysteine, have specific absorbance characteristics at this wavelength. The passage depicts the basic steps for determining the protein extinction coefficient.

• • Overview of Methods for Testing Collagen Content

  Collagen is the main structural protein in various tissues, especially in the skin, bones, tendons, and ligaments. Determining the collagen content can help assess the progression of certain diseases or treatment efficacy, and it can also be used to evaluate the quality of cosmetics and food products.

• • Methods and Uses of Calculating Protein Extinction Coefficient

  The extinction coefficient of a protein describes its absorbance properties under a certain wavelength of light. It is an inherent property of the protein and is linearly related to its concentration and path length. The extinction coefficient is commonly used to estimate the concentration of proteins. The extinction coefficient is defined as the absorbance at a unit concentration and unit path length.

• • Circular Dichroism: Analyzing Alpha Helices' Significance

  Circular Dichroism (CD) spectroscopy is a spectral technique used to study the chirality and secondary structure of molecules, particularly biomacromolecules such as proteins and nucleic acids. The α-helix is a common secondary structure in proteins and is crucial for understanding protein function. CD spectroscopy provides a powerful tool for analyzing α-helix structures in proteins.

• • Antibody Isoelectric Point: Protein Charge Characterization

  The detection of antibody isoelectric point (pI) is an important method for characterizing the charge distribution of proteins. The isoelectric point refers to the pH value at which a protein carries no net charge under an electric potential, i.e. the quantity of positive charges equals the quantity of negative charges.

• • Dynamic Light Scattering Analyzer Testing Steps

  The Dynamic Light Scattering (DLS) analyzer is a device used for measuring the average dynamic diameter of particles such as nanoparticles, proteins, polymers, etc. The test process usually consists of the following basic stages:

• • Antibody Thermal Stability Determination

  Antibody thermal stability determination is to ascertain the stability of an antibody during the heating process, identify its denaturation temperature (Tm), i.e., the temperature point at which the antibody begins to lose structural stability, and evaluate its long-term stability at a specific temperature. The thermal stability of an antibody directly affects its biological activity and functionality, which is crucial for drug development, diagnostic reagents, and research applications.

• • Determination of Protein Triple Helix Structure by Circular Dich

  Circular Dichroism (CD) is a technique commonly used to study protein structure, particularly suitable for measuring the relative content of α-helix, β-sheet, β-turn, and random coil structures in proteins. The triple-helix structure of proteins, especially the triple-helix structure of collagen, can also be analyzed by circular dichroism. In the triple-helix structure of collagen, each helix is composed of three polypeptide chains.

• • How to Analyze Experimental Results of Dynamic Light Scattering?

  Dynamic Light Scattering (DLS) is a common technique used for analyzing the size distribution of nanoparticles, proteins, polymers, etc., in solution. By measuring the change in light scattering intensity over time caused by the Brownian motion of particles in the sample, the particle size can be inferred.