Resources

Proteomics Databases



Metabolomics Databases

• • Can Blood Be Used for Histone Modification

  The feasibility of using blood samples for histone modification research is evident. Blood contains various cell types, including red blood cells, white blood cells, and platelets. For histone modification research, the focus is usually on white blood cells because they contain cell nuclei, and histones are found within the nucleus.

• • Types of Histone Modifications

  Histone modification is an important epigenetic regulatory mechanism, achieved by adding or removing various chemical groups to specific amino acid residues on histones. These modifications can influence the structure and function of chromatin, thereby regulating gene expression. To date, at least nine different types of histone modifications have been discovered.

• • Ubiquitination Sites' Role and Importance in Protein Regulation

  The ubiquitination site of a protein refers to those specific amino acid residues on the protein molecule that can covalently bind with Ubiquitin. Ubiquitin is a small protein that plays an important regulatory role in cells, particularly in protein degradation and signal transduction. Ubiquitination is a post-translational modification process where ubiquitin molecules are attached to specific sites on the target protein.

• • TMT Phosphorylated Protein Proteomics

  TMT (Tandem Mass Tag) Phosphoproteomics is an advanced mass spectrometry analysis technique used to quantitatively analyze changes in protein phosphorylation levels. This technique combines the enrichment of phosphorylated peptides, TMT labeling, and high-resolution mass spectrometry analysis, enabling researchers to perform high-throughput, high-precision analysis of protein phosphorylation across multiple samples.

• • N-Sugar Spectrum Analysis

  N-glycan profiling is a technique used to analyze the structure and composition of protein N-linked glycans. These glycans are a common type of biomolecule widely present in eukaryotes, especially on the cell surface and in secreted proteins. N-glycan profiling is crucial for understanding protein function, cell signaling, cell recognition, and disease mechanisms.

• • Histone Methylation Detection

  Histones are a class of core proteins, mainly found in the cell nucleus of eukaryotes. They bind with DNA to form nucleosomes, thereby organizing DNA compactly into a chromatin structure. The main types of histones include H2A, H2B, H3, and H4, which play a key role in the regulation of gene expression and the protection of cellular genetic information. Histone methylation detection is an experimental method used for research and analysis of histone methylation levels.

• • Acetylation Site Mass Spectrometry Identification

  Acetylation site refers to the specific amino acid residue position on the protein molecule where acetylation modification occurs. Acetylation is a protein modification in which the acetyl group (CH3CO-) is transferred and attached to an amino acid residue in a protein molecule, typically a lysine (lysine) or serine (serine) residue. This chemical modification can regulate the structure and function of proteins, and has a significant influence on cellular processes and signal transduction.

• • How to Determine Ubiquitination Sites

  Ubiquitination is a post-translational modification process in which a small protein called ubiquitin is attached to other proteins. Determining ubiquitination sites is a complex process that can be conducted largely through the following methods:

• • Immunoprecipitation of Phosphorylated Proteins

  Immunoprecipitation (IP) is an experimental technique used for the isolation and purification of specific proteins and their interaction partners, often used in the study of phosphorylated proteins. By using an antibody to specifically bind to the target protein or phosphorylation site, specific proteins or protein complexes can be enriched from complex biological samples. When applied to the study of phosphorylated proteins, this technique is known as phosphorylated protein immunoprecipitation.

• • Tyrosine Phosphorylation Mass Spectrometry

  Tyrosine phosphorylation is one of the key steps in intracellular signal transduction. It leads to the phosphorylation of proteins on tyrosine residues through the action of tyrosine kinase, thereby changing the activity, stability, affinity, or subcellular localization of proteins and thus regulating various cellular functions.