Resources

Proteomics Databases



Metabolomics Databases

• • Workflow of C-Terminal Sequencing

  C-terminal sequencing, a vital technique in proteomics, identifies the amino acid sequence at the C-terminus of proteins. This method is essential for understanding protein structure and function and has numerous applications in biotechnology and medicine. Below is a comprehensive overview of the workflow involved in C-terminal sequencing.

• • Principle of N/C Terminal Sequencing

  N- and C-terminal sequencing are fundamental techniques in molecular biology, offering critical insights into protein structure and function. These methods are essential for studying protein integrity, understanding biological processes, and ensuring the quality of biopharmaceutical products.

• • HDX MS: A New Perspective on Protein Structural Dynamics

  Hydrogen/Deuterium Exchange Mass Spectrometry (HDX MS) is a powerful technique used to study the three-dimensional structure, folding, dynamics, and interactions between proteins or between proteins and other molecules. This method is based on the natural exchange phenomenon between hydrogen atoms and deuterium (an isotope of hydrogen) in the side chains of protein amino acid residues.

• • Mechanism of Nano LC-MS/MS in Protein Sequence Analysis

  Analyzing protein sequences is crucial for understanding their functions, structures, and interactions. Mass spectrometry (MS) technology, especially nano liquid chromatography-tandem mass spectrometry (Nano LC-MS/MS), has become a primary tool in proteomics research.

• • Application of Mass Spectrometry and Edman Degradation in Protein Sequencing

  Proteins are essential functional molecules in living organisms, and studying their structure and function is crucial for understanding biological processes and disease mechanisms. Protein sequencing, the determination of the amino acid sequence of proteins, is a key step in biological research. Currently, mass spectrometry and Edman degradation are the two main methods of protein sequencing.

• • Workflow of Mass Spectrometry-based Protein Sequencing

  Protein sequencing is an essential biotechnological method for determining the amino acid sequence of proteins, crucial for understanding their function, structure, and roles in biological systems. The main steps in protein sequencing methods include sample preparation, protein separation, protein cleavage, peptide separation, peptide identification, and data analysis.

• • Advantages and Disadvantages of Protein Sequencing Techniques

  Proteins are essential molecules that carry out various functions in living organisms. Understanding their structure and function is crucial for deciphering biological processes and disease mechanisms. Advances in protein sequencing techniques have allowed scientists to accurately determine the amino acid sequences of proteins, providing valuable insights for functional studies and biotechnological applications.

• • Principle of Protein Sequencing Methods

  Proteins are essential molecules that perform various functions in living organisms, with their structure being closely linked to their function. Understanding the amino acid sequences of proteins is crucial for studying their functions and roles in biological processes. The primary methods for protein sequencing are chemical degradation and mass spectrometry.

• • Mechanism of Protein Full-Length Sequencing

  Proteins are essential molecules that execute a variety of functions within organisms, including catalysis, structural support, and regulation. Precisely determining the amino acid sequences of proteins is critical for studying their structure and function. Full-length protein sequencing involves multiple methods and steps to analyze complex protein samples comprehensively.

• • Application of Protein Full-Length Sequencing

  Full-Length Protein Sequencing (FLPS) is a technique that allows the comprehensive sequencing of a protein molecule from its N-terminus to its C-terminus. Recent advancements in this technology have opened new avenues in biological research, particularly in understanding protein structure-function relationships, elucidating disease mechanisms, and developing new drugs.