Resources

Proteomics Databases



Metabolomics Databases

• • The Application of TMT Technology in Proteomics

  The Tandem Mass Tag (TMT) quantification technology has a wide range of applications in proteomics. This technique allows researchers to simultaneously quantify proteins in multiple samples using chemically labeled tags. Here are some key examples of the applications of TMT technology: Biomarker discovery: In disease research, TMT technology is used to identify and validate potential biomarkers.

• • Deep Quantitative Proteomics of Biological Samples via iTRAQ/TMT

  iTRAQ (Isobaric Tags for Relative and Absolute Quantitation) and TMT (Tandem Mass Tag) are two commonly used mass spectrometry labeling techniques for protein quantification analysis in proteomics research. These techniques quantify the expression levels of proteins in different samples by using chemical tags, particularly suitable for comparing biological samples under different conditions, such as disease versus normal state, or cells before and after drug treatment.

• • Proteomics of Animal Organizations

  Animal tissues are composed of different types of cells, which have specific structures and functions. Animal tissues include many different types, each with specific physiological functions and characteristics. Common types of animal tissues include muscle tissue, nerve tissue, blood tissue, skin tissue, and adipose tissue, among others. Different types of animal tissues work together to maintain the normal physiological functions of the animal.

• • Whole Blood Sample Collection in Proteomics Analysis

  In proteomics analysis, the collection of whole blood samples is an important step, which is done through venous blood collection and requires the use of sterile techniques to avoid contamination. The collected blood should be immediately labeled and refrigerated to maintain sample integrity and reduce protein degradation. Additionally, the samples should be transported and stored under low temperature conditions until proteinomics analysis is performed.

• • Precautions for Quantitative Proteomics Experiments

  Quantitative proteomics experiments are important biological research methods used to measure the relative or absolute amounts of proteins in different biological samples. These experiments require strict adherence to a series of precautions to ensure the accuracy and reproducibility of the data.

• • Quantitative Shotgun Proteomics

  Quantitative shotgun proteomics is an advanced proteomic technique that allows scientists to simultaneously identify and quantify thousands of proteins in complex biological samples. Quantitative shotgun proteomics relies on the combination of high-performance liquid chromatography (HPLC) and tandem mass spectrometry (MS/MS). Proteins are enzymatically digested into peptides, which are then separated by liquid chromatography and quantitatively analyzed by mass spectrometry.

• • Protein Group Verification

  Protein validation aims to confirm the existence of proteins, identify their characteristics, and understand their roles and interactions in biological processes. This validation method is crucial for gaining a deeper understanding of the roles and functions of proteins in organisms and for advancing biomedical research and drug development.

• • Single-Cell Proteomics Research

  Single-cell proteomics is a field that studies the expression and regulation of proteins within individual cells. This level of resolution provides researchers with a deeper understanding of cellular heterogeneity, microenvironmental influences, and interactions between cells. It complements single-cell genomics and single-cell transcriptomics, together providing a more comprehensive view of cellular-level biological information.

• • SWATH Quantitative Proteomics

  "SWATH" stands for "Sequential Window Acquisition of all Theoretical Mass Spectra," which is a mass spectrometry technique commonly used for quantitative proteomics analysis. Today, I will introduce the basic principles and characteristics of SWATH quantitative proteomics.

• • Antibody Drug Mycoplasma Detection

  Antibody drugs are a type of biological product, commonly used in the treatment of various complex diseases, including autoimmune diseases, inflammatory diseases, and cancer.