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Proteomics Databases



Metabolomics Databases

• • Comparison of iTRAQ and TMT Techniques: Choosing the Right Protein Quantification Method

  The advancement of protein quantification technologies has significantly propelled progress in life sciences, particularly in the investigation of disease mechanisms, biomarker discovery, and drug development. As two widely adopted isobaric labeling-based quantification methods, iTRAQ (Isobaric Tags for Relative and Absolute Quantitation) and TMT (Tandem Mass Tags) are commonly employed for the quantitative analysis of complex biological samples. Each technique exhibits distinct characteristics in terms....

• • Application of Multiple Reaction Monitoring (MRM) in Protein Quantification Analysis

  In life sciences and biomedical research, protein quantification plays a crucial role in understanding biological processes, identifying disease biomarkers, and developing novel therapies. Multiple Reaction Monitoring (MRM), a mass spectrometry-based technique characterized by high specificity and sensitivity, has become a key tool for protein quantification analysis. Overview of Multiple Reaction Monitoring (MRM) Technology 1. What Is Multiple Reaction Monitoring (MRM)? MRM is a mass spectrometry .......

• • Label-Free Quantification Methods: Mass Spectrometry-Based Protein Quantification Strategies

  In contemporary life sciences, protein quantification plays a pivotal role in elucidating physiological functions, understanding disease mechanisms, and identifying drug targets. Unlike methods that only detect the presence of proteins, quantitative approaches provide insight into dynamic changes in protein expression levels, which are critical for biomarker discovery, disease subtype classification, and mechanistic investigations. Mass spectrometry (MS), as a central technology in proteomics, offers two...

• • Step-by-Step Guide to Monoclonal Antibody De Novo Sequencing

  Why Perform De Novo Sequencing on Monoclonal Antibodies? In antibody drug development and research, acquiring the full-length amino acid sequence of an antibody is the essential starting point. However, conventional gene sequencing approaches are often not applicable in the following scenarios: 1. The antibody is only available as a purified protein, with no access to B cells or mRNA; 2. The antibody originates from external sources, literature reports, or commercial suppliers, and lacks original sequence..

• • De Novo Sequencing vs Reference-Based Sequencing: What’s the Difference?

  Different research objectives and sample types demand distinct sequencing approaches. This is especially true in applications such as proteomics or antibody analysis, where researchers often face confusion when choosing between De Novo sequencing and reference-based sequencing: What fundamentally differentiates the two? Under what circumstances is De Novo essential? And why has it become a pivotal technology in current protein structure analysis? This article aims to clarify the distinctions and selection..

• • What Is De Novo Sequencing?

  De Novo sequencing is a technique that reconstructs the original primary structure of a biomolecule—such as DNA, RNA, or proteins—directly from experimental data, without relying on any prior sequence information or reference databases. It serves as a critical approach for investigating novel species, new mutants, unknown antibodies, natural proteins, and other unexplored biological samples. In proteomics, De Novo sequencing involves the use of high-resolution tandem mass spectrometry (MS/MS) to analyze....

• • Introduction to De Novo Sequencing: Principles, Workflow, and Applications

  What Is De Novo Sequencing? De Novo sequencing refers to a method that infers the sequences of biological macromolecules (such as proteins, DNA, or RNA) directly from experimental data, without relying on any known reference sequences or databases. In the field of proteomics, it specifically denotes the process of acquiring MS/MS fragment data of enzymatically digested peptides using high-resolution mass spectrometry, followed by algorithmic analysis of the fragment ions to reconstruct the amino acid.......

• • De Novo Monoclonal Antibody Sequencing: Technical Analysis, Advantages, and Applications

  What Is De Novo Monoclonal Antibody Sequencing? De Novo antibody sequencing is a technique that reconstructs complete monoclonal antibody sequences directly from protein samples, without relying on any reference gene or sequence databases. Using high-resolution liquid chromatography–tandem mass spectrometry (LC-MS/MS), enzymatically digested antibody peptides are analyzed, and algorithms are applied to interpret MS/MS fragment spectra and progressively deduce the corresponding amino acid sequences.

• • Protein Sequence Analysis for Drug Discovery

  What Is Protein Sequence Analysis? Protein sequence analysis involves the systematic comparison, annotation, structural modeling, and functional prediction of amino acid sequences and their characteristic features, such as signal peptides, transmembrane domains, and functional sites. This process typically incorporates several categories of analytical approaches: Sequence alignment (e.g., BLAST, Clustal Omega): used to identify sequences homologous to known proteins, enabling inference of potential ........

• • Amino Acid Sequence Analysis: Techniques and Challenges

  The amino acid sequence forms the foundation of protein function and serves as a crucial entry point for understanding regulatory mechanisms in organisms, disease progression, and the action of molecular drugs. With the rapid advances in proteomics, amino acid sequence analysis has evolved from traditional chemical determination methods to high-throughput, modifiable, and multidimensional data integration approaches, establishing itself as a vital link between genomics and phenotypic function. This paper...