Resources
Proteomics Databases
Metabolomics Databases
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• Isoelectric Focusing Electrophoresis
Isoelectric focusing electrophoresis (IEF) is a technique used to separate proteins or other amphoteric electrolytes according to their isoelectric point (pI). The isoelectric point refers to the pH at which a protein carries no net charge. In this process, samples undergo electrophoresis in a gel with a pH gradient. Under the influence of an electric field, proteins migrate until they reach their isoelectric point, where they stop moving. IEF is an efficient method for separating complex protein mix......
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Protein glycosylation is the process by which glycan moieties are covalently attached to the amino acid side chains of proteins, representing a crucial form of post-translational modification (PTM). This widespread biological phenomenon plays a key role in various biological functions. It is involved in protein folding, stability, and functional regulation, and also influences critical biological processes such as cell recognition, signal transduction, and immune responses. The diversity and complexi......
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Differential Scanning Calorimetry (DSC) is a sophisticated analysis DSC technique used to study the thermal properties of materials. It is widely applied across multiple scientific disciplines, including biochemistry, materials science, and food science. The principle of analysis DSC involves measuring the difference in heat flow between a sample and a reference material as the temperature is varied. This process provides valuable thermodynamic data, allowing researchers to gain insights into phase t......
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Mass spec analysis is a vital tool for determining molecular weights and elucidating compound structures. By measuring the mass-to-charge ratio (m/z) of ionized molecules or atoms, this technique enables researchers to accurately determine the molecular weight, structure, composition, and abundance of compounds. Its high sensitivity, specificity, and rapid analytical capabilities have made mass spec analysis indispensable across multiple fields, including drug development, environmental monitoring, f......
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• Multiple Reaction Monitoring
Multiple reaction monitoring (MRM) is a highly sensitive and specific analytical technique based on mass spectrometry, widely used for targeted analysis of complex biological samples. MRM works by selecting specific ion pairs-combinations of parent ions and product ions—to achieve both quantitative and qualitative analysis of target substances. Compared to traditional mass spectrometry, MRM provides significant advantages in terms of sensitivity and specificity, especially for detecting target analyt......
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• Tandem Mass Spectrometry in Proteomics Research
Tandem mass spectrometry (TMT) is a quantitative analysis technique widely used in proteomics research. TMT enables the relative quantification of multiple samples in mass spectrometry analysis through chemical labeling. This technique uses isotopically labeled chemical reagents that react with peptides in the sample, imparting unique mass tags to each sample. During the mass spectrometry process, these tags generate distinct fragment ion signals, allowing for direct comparison of protein relative ab......
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• TMT Technology in Biomedical and Environmental Research
Tandem Mass Tag (TMT) is a chemical labeling method designed for simultaneous analysis of multiple samples by attaching isotope-labeled tags to proteins from different sources. This approach is widely employed in biomedical research for both relative and absolute protein quantification. The principle of TMT involves introducing isotopic tags onto peptides, which produce distinct ion signals during mass spectrometry analysis. By comparing the intensity of these signals, researchers can precisely deter......
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Stable isotope labeling by cell culture (SILAC) is a technique used to study proteomics, metabolic pathways, and cellular biological processes. SILAC involves labeling cellular proteins with amino acids containing stable isotopes during cell culture, allowing for the distinction of labeled proteins from unlabeled ones in mass spectrometry analysis. This method provides a powerful tool for researchers to quantify proteins, examine protein-protein interactions, and investigate functional changes, with ......
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Cyclic peptide synthesis refers to the chemical or biotechnological processes used to create peptides with a cyclic structure. These cyclic peptides are structurally diverse and exhibit notable biological activity, making them highly applicable in fields such as drug development, agriculture, and biochemical research. For instance, cyclosporine, an important immunosuppressant, is widely used in organ transplants to prevent rejection, while cucurbitacin has demonstrated potential as an anticancer agen......
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• Quantitative Protein Profiling
Quantitative protein profiling integrates mass spectrometry technology with bioinformatics analysis to accurately measure the abundance and changes in protein levels within samples. This technique provides researchers with detailed insights into protein expression in biological samples, which is crucial for understanding cellular processes, disease mechanisms, and drug development. In basic research, quantitative protein profiling enables scientists to uncover dynamic alterations in protein networks ......
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