Resources

Proteomics Databases



Metabolomics Databases

• • Similarities and Differences Between Peptide Database Searching and Protein Database Searching

  The identification of polypeptides in databases is primarily performed by comparing the target polypeptide with entries in the database to ascertain its structural and functional characteristics. This approach relies on mass spectrometry, where the mass spectra of the polypeptides are analyzed, and candidates with matching molecular weights and charge states are identified. Peptide Mass Spectrometry-Based Identification Peptide mass spectrometry-based identification primarily involves aligning target.......

• • Advantages of Spatial Proteomics

  Spatial proteomics is a cutting-edge method for examining protein localization within cells or tissues. By integrating proteomics and advanced microscopy techniques, it allows for a quantitative analysis of the spatial distribution and functionality of proteins. The benefits of spatial proteomics are outlined below.

• • Proteomics Measures Which Proteins

  Proteomics is a field dedicated to the comprehensive study of protein expression, modifications, interactions, and network formation within biological systems. The primary areas of focus include protein expression, structure, function, interactions, modifications, and quantitative protein analysis. The following provides an overview of protein types commonly examined in proteomics research:

• • How to Determine Positive and Negative Peaks in Peptide Mass Spectrometry

  In mass spectrometry analysis, it is crucial to examine the spectrum to identify various peaks, which can manifest as either positive or negative, termed as positive and negative peaks. Positive peaks typically suggest a higher concentration of the substance or a larger ion mass, whereas negative peaks might indicate a lower concentration or smaller ion mass. However, these interpretations are not absolute, as peak values are influenced by numerous factors, including sample characteristics and analytical...

• • Mass Spectrometry Tests the Primary Molecular Weight of Collagen Protein

  In biochemical research, mass spectrometry can be used to measure the size of molecules, determine their composition and even identify unknown substances. In this article, we will explore how to use mass spectrometry to test the primary molecular weight of collagen. Collagen is a macromolecular protein predominantly found within the muscle tissues of animals. Its primary structure comprises amino acids linked by peptide bonds, forming extended chain-like structures. The most critical amino acids for........

• • After Immunoprecipitation, Mass Spectrometry Analysis or Grayscale Analysis Is Usually Performed

  Analysis of post-immunoprecipitation samples is primarily conducted using mass spectrometry or densitometric analysis, each offering distinct advantages and limitations. The choice of method should align with the specific requirements of the study. Mass Spectrometry: Mass spectrometry (MS) is a sophisticated analytical technique used to determine the molecular mass, elemental composition, and chemical structure of samples. In the context of immunoprecipitation studies, MS provides precise identification....

• • Mass Spectrometry Analysis to Identify Downstream Proteins

  Mass spectrometry is a powerful analytical technique utilized to determine the chemical composition and structural information of various substances by measuring the mass or mass-to-charge ratio of ions. This technique finds extensive applications across fields such as food science, environmental science, and biological sciences. In the realm of biological sciences, mass spectrometry is frequently employed to investigate protein structure and function, as well as to identify downstream proteins.

• • NMR Peptide Structure Analysis

  1. NMR Spectroscopy Data Collection: NMR spectroscopy data collection involves acquiring spectra from peptide samples using nuclear magnetic resonance instruments. One-dimensional and two-dimensional spectra are utilized to obtain chemical shift information and associated coupling constants of peptides. 2. NMR Spectra Assignment: In the analysis and assignment of NMR spectra, chemical shifts are assigned to each atom within the amino acid residues. The primary methods employed include nuclear Overhauser....

• • Mass Spectrometry Detection IP Sample Submission

  Introduction to Sample Amount in Mass Spectrometry for IP Samples: Choosing the appropriate sample amount for mass spectrometry analysis of immunoprecipitation (IP) samples is crucial for ensuring experimental accuracy and effectiveness. Here, we provide essential information on selecting the appropriate sample amounts.

• • IPMS Screens for Unknown Interacting Proteins

  Proteins are fundamental to biological processes, often realizing their functions through interactions with other proteins. Hence, identifying protein-protein interactions is crucial for understanding the complex biological activities within organisms. This paper employs Immunoprecipitation Mass Spectrometry (IPMS) to explore unknown protein interactions.