Resources
Proteomics Databases
Metabolomics Databases
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Mascot protein analysis is a widely utilized bioinformatics tool in proteomics for interpreting mass spectrometry data and identifying proteins. This tool functions by comparing experimental mass spectrometry spectra with predicted spectra from known protein databases to identify proteins present in samples. Typically, mass spectrometry data are derived from peptide fragments. Through this approach, researchers can dissect the protein composition of complex biological samples. Mascot's primary role is......
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MALDI-TOF peptide analysis employs matrix-assisted laser desorption/ionization-time of flight mass spectrometry for the analysis of proteins and peptides. In proteomics research, this technique is extensively applied for protein identification, relative quantification, and studying post-translational modifications. The core concept involves using laser energy to desorb and ionize peptides from a solid matrix, followed by mass analysis via a time-of-flight spectrometer. For protein identification, the ......
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MALDI-TOF proteomics is an advanced analytical technology combining matrix-assisted laser desorption ionization (MALDI) and time-of-flight mass spectrometry (TOF) for protein identification and analysis. This technique employs a laser to ionize proteins in a sample and accelerates these ions through an electric field, allowing the measurement of their flight times. As ions of different masses have varying flight times, this method can determine a protein's mass through a time-of-flight spectrum. Recog......
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Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is a technique that utilizes a laser and a matrix to facilitate the desorption and ionization of sample molecules. During MALDI-MS analysis, the sample is mixed with a matrix material, typically a small molecule compound capable of absorbing laser energy to ionize the sample molecules. Upon laser irradiation, the matrix absorbs the energy, initiating the desorption and ionization process, which generates charged ions from the sam......
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MALDI protein analysis is an advanced method based on mass spectrometry that employs a laser as an energy source to ionize proteins from solid to gaseous states via matrix molecule absorption and transfer. This technique is highly valued in proteomics for its minimal sample requirements, rapid analysis, and high sensitivity, making it indispensable for determining protein molecular weight, analyzing structures, and identifying samples. MALDI protein analysis is instrumental in biomedicine, drug develo......
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• Label-Free Quantitative Mass Spectrometry
Label-free quantitative mass spectrometry (LFQ) is a powerful tool in proteomics research, enabling the direct analysis of relative protein abundance changes in samples without requiring exogenous labeling. This method offers significant advantages, including high throughput and cost efficiency, while preserving the intrinsic properties of proteins during analysis. By eliminating labeling steps, LFQ simplifies experimental workflows and minimizes associated errors and complexities. Furthermore, LFQ fa......
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• Isobaric Labeling Mass Spectrometry
Isobaric labeling mass spectrometry is a technique that introduces isotopic labels into specific biomolecules via chemical methods, facilitating quantitative and qualitative analysis using mass spectrometry. This approach finds broad applications in proteomics, particularly in the study of differential proteomics and dynamic protein changes. As biomedical research advances, the need for precise quantification and identification of proteins in complex biological systems is growing. By introducing known......
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• Label-Free Quantification Using MaxQuant
Label-Free Quantification using MaxQuant is a mass spectrometry-based proteomics analysis method primarily utilized for the relative quantification of protein abundance. As the field of proteomics rapidly evolves, there is an increasing demand for precise and efficient methods to analyze complex protein mixtures. Traditional labeling techniques such as iTRAQ and SILAC, while providing accurate quantitative data, require complex chemical labeling of samples, which is both cumbersome and costly. In cont......
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• Identification of Post-Translational Modifications
The identification of post-translational modifications (PTMs) represents a pivotal area in contemporary biological and biochemical research. PTMs refer to a series of chemical changes that proteins undergo following translation, including phosphorylation, acetylation, methylation, ubiquitination, and glycosylation. These alterations can profoundly influence protein function, stability, localization, and interactions with other molecules, thus playing a critical role in processes like cell signaling, m......
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IPA protein analysis is a specialized software tool designed for the analysis and interpretation of complex biological data. It is extensively utilized in proteomics, genomics, and transcriptomics to assist scientists in understanding the functions and mechanisms of biological systems in greater depth. By leveraging the comprehensive data resources of the Ingenuity Knowledge Base, the software provides insights into gene-protein interactions, metabolic pathways, and signaling pathways. The primary fun......
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