Resources

Proteomics Databases



Metabolomics Databases

• • Single-Cell Proteomic Analysis

  Single-cell proteomic analysis aims to investigate the protein composition and dynamics within individual cells, offering detailed biological insights into cellular heterogeneity. Cells are the fundamental units of life, and proteins, as the functional molecules responsible for biological activities, directly influence cellular states and behaviors. In multicellular organisms, even cells of the same type can exhibit significant functional differences, which are often obscured by traditional bulk analy......

• • SILAC Analysis

  SILAC analysis (Stable Isotope Labeling by Amino Acids in Cell Culture) is a powerful technique for quantitative proteomics. By incorporating stable isotope-labeled amino acids into cellular proteins during growth, SILAC analysis enables accurate in vivo protein labeling, facilitating comparative proteomic studies. This method has broad applications in both fundamental and applied research. In fundamental research, SILAC analysis is extensively used to investigate core biological processes such as the......

• • SILAC Quantitative Proteomics

  SILAC quantitative proteomics represents an advanced analytical technique designed to study the dynamic changes of proteins across various biological conditions. This method incorporates stable isotope-labeled amino acids during cell culture, facilitating the precise quantification of target proteins. Specifically, SILAC employs amino acids labeled with stable isotopes such as ^13C and ^15N instead of standard amino acids. Following cell culture, the proteins synthesized are inherently labeled with th......

• • SILAC Phosphoproteomics

  SILAC phosphoproteomics represents an advanced methodology that integrates stable isotope labeling with phosphoprotein analysis to investigate the dynamic alterations of phosphorylated proteins within cells. Phosphorylation is a prevalent post-translational modification, playing critical roles in cellular signaling, metabolic regulation, and cell cycle control. Investigating changes in phosphorylated proteins is crucial for elucidating cellular signaling pathways, disease mechanisms, and drug effects.......

• • Proteomics Differential Expression Analysis

  Proteomics differential expression analysis is a widely used approach for investigating variations in protein expression levels across different biological conditions. The primary goal of this method is to identify differentially expressed proteins in biological samples that arise due to external stimuli, pathological states, or genetic variations. This analytical strategy has broad applications in life sciences, particularly in disease research, drug development, and biomarker discovery.   In disease......

• • Secretome Analysis Mass Spectrometry

  Secretome analysis mass spectrometry is a mass spectrometry-based proteomics approach designed to detect and characterize protein components that are actively secreted or passively released by cells, tissues, or body fluids. Secreted proteins play essential roles in intercellular communication, signal transduction, immune regulation, and disease pathogenesis. Secretome analysis mass spectrometry facilitates the elucidation of functional protein networks in biological systems and contributes to researc......

• • Proteomics Biomarker Analysis

  Proteomics biomarker analysis represents a pivotal methodology in modern life sciences. Biomarkers are quantifiable indicators used to objectively assess biological processes, pathological conditions, or pharmacological responses. In proteomics research, comprehensive protein profiling of biological samples enables the identification of protein biomarkers associated with specific diseases or physiological states. These biomarkers serve as critical tools for early disease diagnosis, prognosis evaluatio......

• • Chemical Crosslinking Mass Spectrometry

  Chemical crosslinking mass spectrometry, an advancing technique within structural biology research, is extensively employed to elucidate protein-protein interactions (PPI) and protein architectures. The fundamental principle involves utilizing chemical crosslinkers to form covalent bonds between specific amino acid residues within interacting proteins or protein complexes, followed by the identification of these linked sites through mass spectrometric analysis to infer the spatial conformation and int......

• • De Novo Peptide Sequencing in Research and Industry

  De novo peptide sequencing allows for the determination of amino acid sequences of unknown peptides without prior sequence knowledge. This method is crucial for analyzing complex protein mixtures, thus aiding scientists in understanding biological structures and functions. In the realm of medical research, it plays a pivotal role in identifying disease-related biomarkers, which are essential for the early diagnosis and treatment of conditions such as cancer, cardiovascular diseases, and neurological d......

• • Chemical Cross-Linking Mass Spectrometry

  Chemical Cross-Linking Mass Spectrometry (CLMS) is an advanced technique in biochemistry and structural biology, combining chemical cross-linking with mass spectrometry to investigate protein-protein interactions and the three-dimensional architecture of protein complexes. The fundamental principle involves using chemical cross-linkers to form covalent bonds between adjacent amino acid residues, thereby preserving spatial information between or within protein molecules. Mass spectrometry then detects ......