Quantitative Amino Acid Analysis Service
- State-of-the-art Quantitative Amino Acid Analysis Service platform
- Extensive expertise in handling samples and analyzing over 100 different amino acids
- Rigorous data analysis and quality control
- Comprehensive report with both raw data, processed data and interpretation of the results
- Competitive pricing and fast turnaround time
- Dry protein samples: ≥ 100 µg, with a concentration of approximately 0.5-2 mg/mL.
- Liquid material: >100 μL at a minimum concentration of 0.5 mg/mL.
The Quantitative Amino Acid Analysis Service provides a precise and reliable method for quantifying amino acids in complex biological samples. The Quantitative Amino Acid Analysis Service employs high-resolution mass spectrometry (MS) techniques, specifically utilizing liquid chromatography-tandem mass spectrometry (LC-MS/MS) platforms. The quantitative amino acid analysis technique allows for the identification and quantification of amino acids at a single-molecule level, making it ideal for analyzing proteins, peptides, and biological fluids with high sensitivity.
The Quantitative Amino Acid Analysis Service is critical for a variety of applications, including the investigation of protein structure-function relationships, the study of enzymatic activities, and the monitoring of metabolic changes in response to environmental or genetic factors. It is also invaluable in protein production processes where amino acid composition can significantly impact product quality and yield. Whether used for basic research, therapeutic development, or industrial applications, this service provides the accurate data required for informed decision-making.
To meet the growing needs of researchers and companies, MtoZ Biolabs now offers this specialized Quantitative Amino Acid Analysis Service. Our Quantitative Amino Acid Analysis Service enables clients to gain in-depth knowledge about the amino acid composition of their biological samples, thereby advancing their research objectives. Free project evaluation, welcome to learn more details!
Analysis Workflow
1. Protein Hydrolysis
Proteins in the sample are hydrolyzed, typically using acid or enzymatic digestion, to release free amino acids for analysis.
2. Impurity Removal
After hydrolysis, the sample undergoes purification to remove impurities and non-amino acid components, ensuring accurate quantification.
3. Derivatization
Amino acids are derivatized, if necessary, to enhance detection sensitivity and improve chromatographic separation during quantitative amino acid analysis.
4. Chromatographic Separation
Derivatized amino acids are separated using high-performance liquid chromatography (HPLC) or liquid chromatography-mass spectrometry (LC-MS), providing high resolution.
5. Mass Spectrometry Quantification
The separated amino acids are analyzed using tandem mass spectrometry (MS/MS) or other advanced MS techniques, offering precise quantification based on amino acid ion mass-to-charge ratios.
6. Data Interpretation
The data is processed and analyzed to determine the amino acid composition and concentration in the sample, delivering accurate results for research or quality control purposes.
Service Advantages
Sample Submission Suggestions
Note: Feel free to contact us if you have any additional sample requirements.
FAQ
Q1: What are the common factors that could lead to inaccuracies in the Quantitative Amino Acid Analysis Service, and what measures are taken to prevent them?
Several factors can lead to inaccuracies in Quantitative Amino Acid Analysis Service, including sample contamination, incomplete hydrolysis, improper derivatization, and matrix effects during chromatographic separation. To minimize these errors, we ensure rigorous sample preparation, use high-purity reagents, and optimize hydrolysis conditions. Impurities are removed using solid-phase extraction, and derivatization protocols are carefully controlled for consistent results. Additionally, the chromatographic separation and mass spectrometry methods are fine-tuned to eliminate matrix effects, ensuring precise and reliable quantification of amino acids.
Q2: What derivatization reagents are commonly used in the Quantitative Amino Acid Analysis Service?
Common derivatization reagents used in Quantitative Amino Acid Analysis Service include phenyl isothiocyanate (PITC), 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC), and o-phthalaldehyde (OPA). These reagents enhance amino acid detection by improving chromatographic behavior and fluorescence, ensuring high sensitivity and accurate quantification in complex biological samples.
Deliverables
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment
4. Data Analysis, Preprocessing, and Estimation
5. Bioinformatics Analysis
6. Raw Data Files
How to order?
