Proteomic Identification of Human Cell Proteins

Cells are the basic units of life, and proteins are the key executors of cell function and structure. Comprehensive identification and functional analysis of proteins are critical for understanding the functions, growth, division, and disease-related aspects of cells. In this regard, mass spectrometry (MS) technology offers powerful tools for proteomics.

 

The Core Role of Mass Spectrometry in Protein Identification

1. Protein and Peptide Fingerprint Recognition

After appropriate enzymatic treatment, proteins extracted from cells can be broken down into thousands of peptides. Through mass spectrometry, these peptides can be precisely identified and traced back to the original proteins.

 

2. Post-Translational Modification Analysis

Mass spectrometry allows researchers to identify post-translational modifications on proteins, such as phosphorylation, acetylation, or ubiquitination. These modifications are critical for cell signal transduction and function.

 

3. Protein Quantification

Using labeled or unlabeled methods, mass spectrometry can provide researchers with information about changes in the abundance of specific proteins within a cell.

 

4. Protein Interaction Analysis

In combination with techniques such as immunoprecipitation, mass spectrometry can reveal interactions between proteins, providing key information for studying protein complexes.

 

Service Advantages

1. Innovative Sample Preparation Method

We use specially designed workflows to extract proteins from human cells, maximizing the preservation of their original state and ensuring the reliability and consistency of the analysis results.

 

2. Precision Mass Spectrometry Analysis

With the help of advanced mass spectrometry instruments, we can detect subtle changes in individual proteins, including site-specific post-translational modifications, which are crucial for understanding the function of proteins and their behavior in complex cellular environments.

 

3. In-Depth Data Interpretation

Our data extends beyond protein identification. Through docking with global databases and using the latest bioinformatics tools, we can uncover networks between proteins, their roles in cell signal transduction, and even their relevance to various human diseases.

 

Application

1. Disease Mechanism Research

By comparing the protein composition and state in healthy cells and disease cells, we can discover the molecular roots of diseases, providing clues for new treatment methods.

 

2. Drug Development

Our technology can identify target proteins for drug action and analyze how drugs affect the status of proteins and signal transduction within cells, which is important for drug design and optimization.

 

3. Personalized Medicine

By analyzing the protein map of an individual, we can help doctors formulate more precise treatment plans, realizing the potential of personalized medicine.

 

Human cell protein mass spectrometry identification is a multi-step process, involving sample preparation, mass spectrometry analysis, and data processing. This technology provides us with valuable information about the types, abundance, and functions of proteins in cells, and is of great significance for fields such as disease mechanism research and drug development. We not only provide technical services, but also serve as your partner in your research journey. Feel free to contact us at any time for more service details!