Protein Sequencing Requirements for the Sample

The requirements for samples in protein sequencing usually include the following aspects:

 

1. Sample Purity

The purity of the protein in the sample is crucial. Excessive impurities may interfere with the sequencing results, so various methods (such as centrifugation, filtration, purification, etc.) are usually needed to enhance the purity of the protein.

 

2. Sample Quantity

Different protein sequencing techniques require different amounts of sample. Some methods may require more protein (such as a few micrograms to milligrams), while new high-sensitivity techniques may require only a tiny amount of sample.

 

3. Sample Integrity

The integrity of the protein is crucial for accurate sequencing. The protein in the sample should not be overly degraded or modified.

 

4. Sample Processing

The processing of the sample during extraction and storage is also crucial. It's necessary to avoid protease contamination and store proteins under appropriate conditions to prevent degradation or inactivation.

 

5. Protein Modification

If the focus of the study is protein post-translational modifications (such as phosphorylation, glycosylation, etc.), it's necessary to ensure these modifications are preserved during sample processing.