Protein Mass Spectrometry Service

Protein mass spectrometry service is a specialized analytical service based on advanced mass spectrometry techniques. It enables precise characterization of protein molecular weight, amino acid sequences, post-translational modifications (PTMs), and protein-protein interactions using high-precision mass spectrometers. Widely applied in disease mechanism research, biomarker discovery, and drug development, this service serves as a vital tool in modern life sciences research.

 

Service at MtoZ Biolabs

MtoZ Biolabs leverages industry-leading mass spectrometry platforms, such as the Thermo Fisher Orbitrap Fusion Lumos and Q Exactive HF, combined with Nano-LC systems, to provide efficient and accurate protein mass spectrometry service. From protein identification to complex proteome analysis, our service is designed with optimized workflows covering every step from sample preparation to data analysis, ensuring comprehensive scientific support for our clients.

 

Our protein mass spectrometry service focuses on comprehensive protein characterization and functional studies, including the following key areas:

1. Protein Identification: Quickly and accurately determine the identity of unknown proteins using mass spectrometry.
2. Post-Translational Modification Analysis: Detect multiple PTMs, such as phosphorylation, glycosylation, and acetylation, to unravel mechanisms of protein function regulation.
3. Quantitative Proteomics: Conduct relative or absolute quantification of protein abundance using labeled or label-free methodologies.
4. Protein Interaction Analysis: Reveal protein complexes and their dynamic changes through techniques such as Pull-Down assays and Co-Immunoprecipitation (Co-IP) combined with mass spectrometry.

 

Analysis Workflow

Our protein mass spectrometry service follows an optimized workflow to ensure high-quality results. This process includes sample extraction and purification, enzymatic digestion, peptide separation, mass spectrometry analysis, and data interpretation. Utilizing Nano-LC systems and high-sensitivity mass spectrometers, MtoZ Biolabs delivers precise analysis of complex samples, rapid identification of protein identities, PTM site localization, and comprehensive quantification. Advanced algorithms and software ensure the reliability and reproducibility of results.

• Sample Preparation: Proteins are extracted from client-provided samples, such as cells, tissues, or biofluids.
• Protein Digestion: Proteins are enzymatically cleaved into peptides using reagents like trypsin.
• Separation and Analysis: Nano-LC systems are employed for high-resolution peptide separation.
• Mass Spectrometry Detection: High-sensitivity instruments, such as Orbitrap Fusion Lumos, capture peptide mass data.
• Data Interpretation: Protein identification and PTM analysis are performed using advanced software and database searches.

 



Figure1. Workflow of Protein Mass Spectrometry Service

 

Applications

Protein mass spectrometry service has extensive applications with significant implications across various research domains. Mass spectrometry data empower researchers to gain deep insights into protein functions:

• Disease Mechanism Research: Uncover dynamic protein changes involved in pathological processes.
• Biomarker Discovery: Identify potential diagnostic and therapeutic targets.
• Drug Mechanism Studies: Analyze drug targets and related signaling pathways.
• Signal Pathway Studies: Construct protein interaction networks and decode molecular regulatory mechanisms.
• Biopharmaceutical Development: Support quality assessment and production optimization of antibody therapeutics.

 

What Could be Included in the Report?

1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment (project-dependent)
4. Data Analysis, Preprocessing, and Estimation (project-dependent)
5. Bioinformatics Analysis
6. Raw Data Files

 

Choosing MtoZ Biolabs for protein mass spectrometry service ensures access to high-quality data and professional support. Our experienced technical team tailors service solutions to meet specific project needs, providing optimal answers to every scientific question. If you have additional needs or inquiries, feel free to contact us. We are committed to supporting your research projects and driving breakthrough discoveries.