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    Plant Leaf Proteome N-Glycosylation Analysis

      N-glycans, also known as N-linked glycans, are sugar chains that bind to proteins through nitrogen atoms (usually the amino side chain of an amino acid). In plants, this glycosylation process mainly occurs in the endoplasmic reticulum and Golgi apparatus, and is a key factor in protein folding and stability, and in regulating plant physiological activities. By analyzing the N-glycan structure of plant proteins, researchers can reveal plant response mechanisms to environmental stresses, such as drought, salinity, and cold. In addition, this analysis also helps us understand plant immune responses, as well as how to improve crop adaptability and yield through genetic engineering.

       

      With advanced analytical technologies, including MALDI TOF MS and/or UHPLC, MtoZ Biolabs provides accurate N-glycan analysis services for plant leaf proteins, helping researchers and producers ensure the high quality and biological activity of protein products.

       

      Service Advantages

      1. Advanced Analytical Equipment

      With MALDI-TOF MS and UHPLC technology, we can perform comprehensive N-glycan analysis of proteins in plant leaves. These technologies support all stages from basic research to commercial production, providing key data support.

       

      2. Customized Sample Processing Workflow

      Given the specificity of plant leaf proteins, our workflow includes protein digestion, deglycosylation, purification, derivatization, and final analysis. This process ensures efficient and accurate extraction and analysis of N-glycans from complex biological samples.

       

      3. Accurate Data Interpretation

      Combining mass spectrometry data with advanced bioinformatics tools, we can provide detailed information about N-glycan modifications, including glycan type, structure, and relevance to protein function.

       

      Applications

      1. Biopharmaceutical Development

      Ensuring that the N-glycosylation of plant-derived biopharmaceuticals (such as antibodies and vaccines) meets expectations, to ensure their biological activity and stability.

       

      2. Plant Pathology Research

      Analyzing the changes in N-glycan types of plant leaf proteins, revealing the molecular mechanisms of plant diseases.

       

      3. Crop Improvement Research

      Analyzing the glycosylation pattern of crop proteins, providing insights at the molecular level for crop genetic improvement.

       

      Analysis Workflow

      1. Sample Preparation

      Receive and test the plant leaf samples provided by the customer, confirm that they are suitable for further protein extraction and analysis.

       

      2. Protein Extraction and Digestion

      (1) Use optimized methods to extract proteins from leaves and perform digestion, preparing for the deglycosylation step.

      (2) N-glycan release and purification: Use PNGase F enzyme to release N-glycans, and purify them using specific chromatography techniques.

       

      3. N-Glycan Labeling and UHPLC Analysis

      After derivatization of N-glycans, perform high-performance liquid chromatography analysis using HILIC-UHPLC.

       

      4. Data Analysis and Reporting

      Provide a comprehensive analysis report, including N-glycan spectra, structure identification, and interpretation of its biological significance.

       

      MtoZ Biolabs is committed to providing each customer with fast, accurate, and reliable services, ensuring that your research and production activities can rely on accurate protein N-glycan analysis data. With many years of experience in glycomics analysis services, MtoZ Biolabs can customize glycomics analysis services according to your specific needs. Inquiries are welcome!

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