Parallel Reaction Monitoring Mass Spectrometry
Parallel reaction monitoring mass spectrometry is an advanced quantitative proteomics technique based on high-resolution and high-mass accuracy mass spectrometry, widely employed for the sensitive and specific detection of proteins or peptides. Originating from selected reaction monitoring (SRM), PRM utilizes high-resolution instruments such as the Orbitrap for analysis. Compared to SRM, parallel reaction monitoring mass spectrometry boasts superior specificity and resolution, enhancing the precise detection of target peptides and facilitating the quantification of low-abundance proteins in complex biological matrices. A core advantage of PRM is its capability to simultaneously profile multiple target proteins in a single experiment, achieving high-precision quantification without reliance on standards, thus offering robust data support for biomedical research, biomarker discovery, and drug development.
In the realm of biomedical research, parallel reaction monitoring mass spectrometry is extensively applied to discover and validate disease biomarkers. As contemporary medical studies increasingly investigate the link between proteomic alterations and diseases, PRM emerges as a vital tool for accurately measuring the expression levels of disease-associated proteins. For instance, in cancer research, alterations in the expression of specific proteins may be intricately linked to cancer initiation and progression. PRM enables precise detection of these proteins' abundance variations in clinical samples, providing a foundation for early diagnosis and personalized therapy. Furthermore, PRM is utilized in investigating cardiovascular, neurodegenerative, and autoimmune diseases to screen and confirm potential protein biomarkers.
Beyond biomedical research, parallel reaction monitoring mass spectrometry holds significant value in drug development and targeted therapy investigations. Accurate quantification of target protein abundance and modification status is critical in drug screening and mechanism studies. PRM offers highly sensitive targeted protein quantification, assisting scientists in evaluating drug effects on target proteins and optimizing candidate drug selection strategies.
The operational principle of parallel reaction monitoring mass spectrometry parallels that of SRM, both employing tandem mass spectrometry (MS/MS) to screen and quantify target peptides. However, while SRM depends on triple quadrupole mass spectrometers, selecting target peptides in MS1 and monitoring specific fragment ions (transitions) in MS2, it can be susceptible to background noise and non-specific interference. In contrast, PRM uses high-resolution, high-mass accuracy mass spectrometers like the Orbitrap, enabling simultaneous detection of all fragment ions in MS2, thereby enhancing the accuracy of peptide quantification and minimizing non-specific signal interference.
Precision in quantitative analysis is paramount in proteomics research. The high resolution and mass accuracy of parallel reaction monitoring mass spectrometry ensure accurate differentiation of target peptides, making it particularly adept at detecting low-abundance proteins in complex samples. For example, in plasma, urine, and tissue extracts, where key protein abundance is exceedingly low, PRM improves the reliability of quantification against a backdrop of significant interference. Additionally, PRM can detect post-translational modifications (PTMs) such as phosphorylation, acetylation, and glycosylation, providing a robust approach for studying protein functional regulation mechanisms.
Leveraging cutting-edge mass spectrometry technology and extensive proteomics expertise, MtoZ Biolabs offers high-quality parallel reaction monitoring mass spectrometry services. Our PRM platform enables precise quantification of specific proteins or peptides, extensively applied in disease biomarker research, drug target validation, and post-translational modification analysis.
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
Related Services
How to order?
