Olink Proximity Extension Assay
The olink proximity extension assay is an advanced proteomics technology designed for accurate and efficient protein quantification. Based on the principle of proximity extension, this method integrates high-throughput microfluidic systems with a highly sensitive real-time quantitative PCR (qPCR) detection system. This combination enables the simultaneous detection of multiple target proteins within complex biological samples. The assay is distinguished by its high specificity and sensitivity, allowing for the quantification of hundreds of proteins from a single sample, providing researchers with a wealth of biological insights. Its unique strength lies in combining antibody-based capture specificity with nucleic acid amplification sensitivity, making it a valuable tool in modern proteomics research.
The applications of the olink proximity extension assay span a wide range of fields, including fundamental biological research, clinical studies, drug development, and public health. In basic research, it serves as a powerful tool for investigating cellular signaling pathways, metabolic networks, and disease mechanisms. It is particularly instrumental in biomarker discovery and validation, facilitating the identification of disease-associated protein expression changes and providing crucial data for disease diagnosis and personalized therapy. In clinical research, the assay is widely employed in large-scale epidemiological studies, enabling the analysis of protein expression variations across populations to elucidate disease pathogenesis and progression. Furthermore, in drug development, its high throughput and precision contribute to robust data generation for drug screening and efficacy assessment.
The core principle of the olink proximity extension assay is its proximity extension technology, which utilizes a pair of probes that specifically bind to the target protein. Upon binding, these probes form a proximal double-stranded structure. A primer is then introduced, followed by PCR amplification and detection, ensuring precise quantification of the target protein. The use of multiple probes and amplification steps significantly enhances both specificity and sensitivity while effectively minimizing background noise and non-specific signals.
In practical applications, the olink proximity extension assay offers a streamlined and highly efficient analytical solution. Its standardized workflow and fully integrated detection system allow for high-throughput sample analysis in a short time, making it particularly advantageous for large-scale epidemiological studies. Additionally, the assay requires minimal sample input and does not necessitate complex sample preparation, making it an ideal choice for research projects demanding rapid and reliable results.
MtoZ Biolabs is committed to delivering high-quality Olink proteomics analysis services, supporting researchers in efficiently conducting proteomics studies. Our team of experts possesses extensive experience in handling and analyzing various biological samples. Through our specialized services, clients can obtain comprehensive and reliable proteomic data to advance their research and development projects. We welcome collaboration with research institutions and enterprises to drive progress in life science research.
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
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