Oligonucleotide Sequence Analysis Service

Oligonucleotide sequence analysis is a biotechnological method for identifying the types and sequences of nucleotides in short DNA or RNA fragments (typically 20-30 nucleotides). This technique plays a critical role across numerous biological and medical disciplines. Oligonucleotides have become a focal point in pharmaceutical research, with certain sequences that uniquely bind to or cleave disease-causing genes being developed into novel therapeutics. These oligonucleotides offer high efficiency and a broad application spectrum, complementing traditional medications with promising prospects for widespread use. Oligonucleotide sequence analysis is an essential step in the research and production of gene therapeutics.

 



                                                                                              Christina, J. et. al. Anal. Chem. 2022.

Figure 1. Oligonucleotide Sequence Analysis Process

 

Advancements in mass spectrometry have greatly enhanced the identification of oligonucleotide sequences. Within high-resolution mass spectrometers, oligonucleotide molecules are broken down into fragment ions with specific mass-to-charge (m/z) ratios. Analyzing these ions allows for the deduction of the original nucleotide sequences. MtoZ Biolabs, utilizing Thermo's Orbitrap Fusion Lumos mass spectrometer in conjunction with Nano-LC nanoscale chromatography, has developed a method for precise oligonucleotide sequence analysis. This technique enables detailed identification of various oligonucleotide types, including ASOs, siRNAs, miRNAs, and Aptamers, thereby satisfying a range of customer needs.

 

Deliverables

MtoZ Biolabs provides a comprehensive technical report that includes:

1. Experimental Procedures

2. Relevant Mass Spectrometry Parameters

3. Detailed Information on Oligonucleotide Sequence Analysis

4. Mass Spectrometry Images

5. Raw Data