Oligonucleotide Sequence Analysis Service

    Oligonucleotide sequence analysis is a biotechnological method for identifying the types and sequences of nucleotides in short DNA or RNA fragments (typically 20-30 nucleotides). This technique plays a critical role across numerous biological and medical disciplines. Oligonucleotides have become a focal point in pharmaceutical research, with certain sequences that uniquely bind to or cleave disease-causing genes being developed into novel therapeutics. These oligonucleotides offer high efficiency and a broad application spectrum, complementing traditional medications with promising prospects for widespread use. Oligonucleotide sequence analysis is an essential step in the research and production of gene therapeutics.

     

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                                                                                                  Christina, J. et. al. Anal. Chem. 2022.

    Figure 1. Oligonucleotide Sequence Analysis Process

     

    Advancements in mass spectrometry have greatly enhanced the identification of oligonucleotide sequences. Within high-resolution mass spectrometers, oligonucleotide molecules are broken down into fragment ions with specific mass-to-charge (m/z) ratios. Analyzing these ions allows for the deduction of the original nucleotide sequences. MtoZ Biolabs, utilizing Thermo's Orbitrap Fusion Lumos mass spectrometer in conjunction with Nano-LC nanoscale chromatography, has developed a method for precise oligonucleotide sequence analysis. This technique enables detailed identification of various oligonucleotide types, including ASOs, siRNAs, miRNAs, and Aptamers, thereby satisfying a range of customer needs.

     

    Deliverables

    MtoZ Biolabs provides a comprehensive technical report that includes:

    1. Experimental Procedures

    2. Relevant Mass Spectrometry Parameters

    3. Detailed Information on Oligonucleotide Sequence Analysis

    4. Mass Spectrometry Images

    5. Raw Data

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