Oligonucleotide Purity Analysis Service

Oligonucleotides, including DNA and RNA, have a wide range of uses in gene therapy, biological probes, and diagnosis. For example, DNA can be introduced into immune cells and be modified to express chimeric antigen receptor proteins, which can be used for cell-based immunotherapy. When oligonucleotides are synthesized by solid-phase synthesis techniques, trace impurities may be produced at various stages of the synthesis process. Therefore, the analysis and detection of oligonucleotide purity are particularly important to ensure the quality, accuracy, safety, and biological activity of oligonucleotide in its production and research processes.

 



Kanavarioti, A. et al. Sci Rep. 2019.

Figure 1. Ion-pair Reversed-phase High-performance Liquid Chromatography  (IP-RP-HPLC) Oligonucleotide Purity Analysis

 

SDS-PAGE serves as a conventional method for purifying oligonucleotides by size. However, higher-resolution chromatography is required when oligonucleotides are similar size or contain modifications that hinder separation. The preferred method for oligonucleotide purification is ion-pair reversed-phase high-performance liquid chromatography (IP-RP-HPLC), which can be coupled with MS for detailed oligonucleotide mass characterization. At MtoZ Biolabs, we utilize a HPLC platform to develop and validate methods for analyzing oligonucleotide purity. These methods cater to various oligonucleotides, allowing for the identification of impurities caused by imcomplete reaction, such as n-1 deletions, n+1 additions, incomplete sulfations, and byproducts like diphosphates. Welcome to contact to learn more about our service.

 

Sample Submission Requirements

1. Synthetic Oligonucleotides: Oligonucleotides such as PCR Primers and Probes

2. Natural Oligonucleotides: Oligonucleotides Isolated from RNA or DNA

3. Oligonucleotide Pharmaceuticals: Oligonucleotides Used in Gene, Antiviral, and Anticancer Therapies

 

Deliverables

1. Experimental Procedures

2. Relevant Mass Spectrometry Parameters

3. Detailed Information on Oligonucleotide Purity Analysis

4. Mass Spectrometry Images

5. Raw Data