Nanopore Protein Sequencing Service

The Nanopore Protein Sequencing Service enables direct sequencing of single protein molecules, identifying their amino acid sequences, structural features, and variations. It is particularly suitable for low-abundance proteins, complex biological samples, and studies of protein-protein interactions. This technology is widely applied in the detection of single-molecule nucleic acids, proteins, and nucleic acid-protein complexes, and can even be used for the identification of individual DNA bases and protein amino acids.

 



Sun, H. et al. Anal Chim Acta. 2023.

Nanopore Single-Molecule Biosensor in Protein Denaturation Analysis

 

Nanopore protein sequencing technology is based on a tiny pore structure. When a charged protein molecule passes through this pore, it induces changes in the electrical current. By meticulously analyzing these current signals, the amino acid sequence and structural characteristics of the protein molecule can be identified. The core advantage of this process lies in its single-molecule detection capability, which means that researchers can obtain precise sequence information directly from the original sample without the need for complex separation and processing steps. Additionally, the Nanopore Protein Sequencing Service can detect the amino acid sequence of individual protein molecules and even identify structural variations and functional states of proteins at the molecular level—capabilities that are unattainable with traditional mass spectrometry technologies.

 

Service at MtoZ Biolabs

Leveraging the latest nanopore sequencing technology, MtoZ Biolabs offers an efficient and precise Nanopore Protein Sequencing Service designed to reveal the complete sequences of proteins through real-time single-molecule detection. This service helps clients gain an in-depth understanding of protein structures, functions, and interactions. Our Nanopore Protein Sequencing Service is particularly suitable for a variety of applications, including but not limited to:

✅ Protein Structure Analysis

Gain a deep understanding of the primary structure of proteins and their variations.

 

✅ Protein-Protein Interaction Studies

Reveal the molecular-level interaction mechanisms by directly sequencing the amino acid sequences of proteins.

 

✅ Drug Development and Biomarker Discovery

Accelerate target identification and preclinical validation, providing precise support for drug discovery.

 

✅ Quantitative Analysis of Complex Biological Samples

Efficiently analyze complex biological samples through high-throughput analytical techniques.

 



Branton, D. et al, Nat Biotechnol. 2008.

 

 

Service Advantages

✅ High Throughput and Low Cost

Nanopore protein sequencing utilizes single-molecule detection technology, allowing for the simultaneous processing of a large number of samples and significantly increasing data acquisition throughput. Compared to traditional methods, it is more cost-effective and efficient, making it suitable for large-scale research and industrial applications.

 

✅ Real-Time Data Analysis

Real-time capture of current changes as molecules pass through the nanopore enables rapid analysis and timely data acquisition, accelerating the research process.

 

✅ High Sensitivity and High Accuracy

Capable of identifying single-molecule protein sequences and detecting extremely low-abundance proteins, it is suitable for complex biological samples and offers exceptional sensitivity and accuracy.

 

✅ Label-Free Analysis

No complex labeling or modification is required, avoiding label-induced errors and allowing direct analysis of native protein molecules, outperforming traditional mass spectrometry analyses.

 

Case Study

Resolving Long-Chain Protein Single-Molecule Sequences through Nanopore Protein Sequencing

Protein sequence analysis is crucial for understanding protein function, but traditional methods face limitations in sequencing long-chain proteins and identifying post-translational modifications. This study employed nanopore protein sequencing technology, introducing a multi-pass single-molecule reading approach to accurately decode long-chain protein sequences. By detecting the electrical current signals as proteins pass through the nanopore and combining multi-pass strategies with algorithm optimization, the sequencing accuracy and signal-to-noise ratio were significantly enhanced. The results demonstrated that this method successfully achieved high-precision sequencing of long-chain proteins, effectively reducing error rates and enabling the identification of complex post-translational modifications (PTMs) and structural variants. The research proved that multi-pass nanopore sequencing technology offers a novel solution for protein sequencing, overcoming the limitations of traditional methods and showcasing broad application potential in biomedical research.

 



Motone, K. et al. bioRxiv [Preprint]. 2023.

Rereading Single Protein Molecules Multiple Times with an Unfoldase Slip Sequence

 

FAQ

1. How can high background signals be mitigated in Nanopore Protein Sequencing?

High background signals can be reduced by optimizing nanopore design, adjusting buffer composition, and fine-tuning current parameters. Additionally, employing advanced signal processing algorithms can extract target signals from the noise, thereby enhancing sequencing accuracy.

 

2. How can the issue of long protein chains clogging the nanopore be addressed?

Optimizing protein sample preparation (such as chemical modifications to reduce aggregation), improving nanopore surface coatings, and adjusting experimental conditions (like electric field strength) can effectively minimize the risk of clogging, thereby improving sequencing continuity and efficiency.

 

MtoZ Biolabs' Nanopore Protein Sequencing Service provides a cutting-edge, efficient, and precise tool for proteomic analysis. Whether you are engaged in fundamental research, drug development, or clinical studies, our Nanopore Protein Sequencing Service can help you overcome research bottlenecks and deliver profound biological insights. Leveraging our advanced nanopore sequencing platforms and professional technical team, MtoZ Biolabs offers comprehensive technical support for your research endeavors. Contact us today to explore how our Nanopore Protein Sequencing Service can accelerate your research progress.