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    Multi-Channel Phosphorylated Protein Analysis Service

      Protein phosphorylation, a pivotal post-translational modification (PTM), regulates protein activity within cellular signaling networks. Traditional phosphorylation research methods often fall short in sensitivity and specificity, failing to satisfy the requirements for high-throughput, sensitive studies. Multi-channel phosphorylated protein analysis significantly enhances the efficiency and precision of phosphorylated peptide enrichment through specialized multi-channel kits, allowing for the analysis of thousands of phosphorylation sites per experiment, thereby profoundly advancing proteomic research.

       

      MtoZ Biolabs employs the cutting-edge Orbitrap Fusion Lumos mass spectrometer, coupled with nano-liquid chromatography, to offer integrated multi-channel phosphorylated protein analysis services. You need only specify their requirements and submit their samples; we manages all subsequent processes, with free consultations available.

       

      Analysis Workflow

      1. Protein Extraction

      Optimized techniques are used to extract proteins from a variety of biological samples.

       

      2. Protein Digestion

      Enzymatic digestion is employed to cleave proteins into peptides.

       

      3. Phosphorylated Peptide Enrichment

      Utilizing our advanced multi-channel enrichment kits for the effective enrichment of phosphorylated peptides.

       

      4. Peptide Separation

      High-Performance Liquid Chromatography (HPLC) separates the enriched peptides.

       

      5. Mass Spectrometry Analysis

      Peptides are quantified and identified using high-resolution mass spectrometry.

       

      6. Bioinformatics Analysis

      Comprehensive data analysis elucidates the biological implications of phosphorylation events.

       

      1805902667835887616-蛋白磷酸化检测.png 

      Pisitkun, T. et al. J. Physiol. 2007.

      Figure 1. Analysis Workflow of Phosphorylated Protein

       

      Service Advantages

      High Precision: Enables the accurate identification and quantification of thousands of phosphorylation sites.

       

      High Throughput: Capable of analyzing several thousand phosphorylation sites in a single experiment, ideal for extensive research studies.

       

      High Sensitivity: Effective detection and analysis of even low-abundance phosphorylated peptides.

       

      Quick Turnaround: The entire workflow, from sample receipt to report generation, is completed within a few days.

       

      Sample Submission Requirements

      We accept a diverse range of biological samples, including tissues, cells, blood, and plant materials. The size and preparation of samples are tailored to the design and objectives of each experiment to ensure efficiency and precision.

       

      Deliverables

      1. Experimental Procedures

      2. Relevant Mass Spectrometry Parameters

      3. Detailed Information on Multi-Channel Phosphorylated Protein Analysis

      4. Mass Spectrometry Images

      5. Raw Data

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