mRNA 3’ poly(A) Tail Analysis Service
In eukaryotic cells, the 3' end of mRNA is often extended with a poly(A) tail, a sequence of purine nucleotides. The poly(A) tail is a long chain of adenosine nucleotides of variable length. This tail is crucial for mRNA stability, transport, and translation. The analysis of mRNA's 3' poly(A) tail is a vital technique in studying the polyadenylation at mRNA molecules' 3’ ends, with significant implications for gene regulation, cell development, and disease research. This analysis aids in understanding mRNA processing, stability, transport, and post-transcriptional regulatory processes. Additionally, it is instrumental in drug development and disease diagnostics, enhancing our understanding of disease mechanisms and informing therapeutic strategy design.
Gilar, M. et. al. Anal. Chem. 2023.
Figure 1. mRNA Molecule With a 3’ Poly(A) Tail
Utilizing endonuclease and liquid chromatography-mass spectrometry (LC-MS) technologies, the analysis includes RNAse T1 enzyme digestion and oligo dT magnetic bead purification to isolate the poly(A) tail, followed by qualitative and quantitative assessment via LC-MS. This approach is also a commonly used polyA tailing analysis method in the current in vitro mRNA preparation process. MtoZ Biolabs employs the Orbitrap Fusion Lumos mass spectrometer and Nano-LC chromatography to offer precise, comprehensive mRNA 3’ poly(A) tail analysis services. Free project evaluation, Welcome to learn more details!
Deliverables
MtoZ Biolabs provides a detailed technical report, which includes:
1. Experimental Procedures
2. Relevant Mass Spectrometry Parameters
3. Comprehensive mRNA 3’ Poly(A) Tail Analysis
4. Mass Spectrometry Images
5. Raw Data
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