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    Metabolic Pathways Analysis

      "Metabolic Pathway Analysis" refers to obtaining a metabolite spectrum through mass spectrometry, then using bioinformatics tools to statistically analyze the data, identifying differential metabolites, determining their positions in the metabolic network based on the structure and biological functions of the metabolites, and ultimately revealing their potential metabolic pathways. The main goals of metabolic pathway analysis are:

      • Identifying Key Metabolites: Recognizing critical metabolites within various biochemical pathways is crucial for studying diseases, drug metabolic reactions, and cellular function regulation.
      • Depicting Metabolite Flow: This process helps trace the transfer of metabolites across different cellular tissues and pathways.
      • Revealing Regulatory Mechanisms: Studying the regulatory mechanisms of specific enzymes and metabolic reactions.
      • Providing Biological Insights: Offers a panoramic view of an organism's metabolic state, aiding research, diagnostics, and therapy development.


      As metabolomics research deepens, the demand for metabolic pathway analysis continues to grow. Serving as an important bioinformatics tool, metabolic pathway analysis is crucial for a deeper understanding of biological metabolic processes, discovering disease markers, and developing new drugs.



      Wang, L. et al. Sci Rep. 2019.

      Figure1. polyP Metabolism and Pathway Distributions


      Services at MtoZ Biolabs

      MtoZ Biolabs provides comprehensive metabolic pathway analysis solutions for a variety of biological samples, including blood, urine, saliva, tears, and animal and plant tissues. Our services cover key biochemical pathways such as amino acid metabolism, the citric acid cycle, glycolysis, nucleic acid metabolism, and hormone metabolism, including but not limited to:

      1. Targeted Metabolomics: Detailed analysis of specific metabolites, suitable for studies of predefined metabolite groups, ensuring high sensitivity and accuracy.
      2. Non-targeted Metabolomics: Comprehensive analysis of all detectable metabolites in a sample, suitable for obtaining a panoramic view of the biological system's metabolic information.
      3. Enzyme Kinetics Analysis: Assessing the activity and kinetic properties of enzymes, gaining insight into reaction rates and substrate specificity.
      4. Specific Pathway Enzyme Activity Measurement: Analyzing enzymes in specific biochemical pathways.
      5. Stable Isotope Tracing: Tracing the transformation of labeled substrates in biological systems, revealing metabolic flux rates and pathways.
      6. Flux Balance Analysis: Simulating metabolic fluxes in cellular systems, predicting the impact of substrate changes, optimizing biotechnological processes.
      7. Pathway Enrichment Analysis: Identifying key metabolic pathways in high-throughput omics data.
      8. Visualization and Interpretation: Clearly presenting and explaining metabolic pathways.


      Experimental Instruments

      1. Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

      High-resolution analysis suitable for metabolite analysis, biomarker discovery, and drug metabolism studies.

      2. Gas Chromatography-Mass Spectrometry (GC-MS)

      Analyzing small volatile compounds, suitable for environmental monitoring and chemical analysis.

      3. Nuclear Magnetic Resonance (NMR)

      Non-destructively resolving metabolite structures, used for structural determination of organic compounds and protein-ligand interaction studies.

      4. Ultra-High Performance Liquid Chromatography (UHPLC)

      Used for high-throughput analysis of complex samples, suitable for quantitative analysis of various metabolites and separation of biomolecules.


      Service Advantages

      • High Sensitivity, High Accuracy: Mass spectrometry can accurately identify and quantify metabolites, providing reliable data support for subsequent metabolic pathway analysis.
      • Wide Application Range: Capable of analyzing both individual species and complex organisms.
      • Intuitive and Content-Rich Results: Revealing the metabolic processes of organisms at the molecular level, offering new insights for disease diagnosis and treatment.


      MtoZ Biolabs is committed to providing the highest quality metabolic pathway analysis solutions through advanced technology and personalized services. If you have any needs for metabolic pathway analysis, please feel free to contact us for more technical support.

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