Metabolic Flux Analysis Service

    Metabolic flux analysis (MFA) is a critical technique for examining the production and consumption rates of metabolites in biological systems. In systems metabolic engineering, MFA helps understand cell physiology under specific conditions and predict metabolic capacities of cells following genetic or environmental changes. MFA is also used to elucidate cellular mechanisms. By using stable isotope labeling (such as 2H, 13C, and 15N), MFA can track isotopic labels along metabolic pathways, thereby identifying and quantifying the roles of these pathways. Methods such as 2H and 13C NMR, along with mass spectrometry, are commonly used in MFA. Tandem mass spectrometry, a newer technique, offers high-resolution quantification of metabolic fluxes in complex biological systems.



    Guo, W. et al. Bioengineering (Basel). 2015.

    Figure 1. Metabolic Flux Analysis Services


    MtoZ Biolabs offers a MFA platform that analyzes control structures and regulatory networks of metabolic pathways. This platform is suitable for any metabolic system that can be established by kinetic models.


    Analysis Workflow

    1. Experimental Design: We provide customized experimental designs based on your research subjects and objectives.

    2. Sample Analysis: We offer various analyses of metabolic fluxes, including glucose metabolic flux, glutamine flux, and tricarboxylic acid cycle (TCA cycle) metabolic flux.

    3. Data Analysis: Our metabolomics analysis includes processing mass spectrometry raw data, analyzing the data, quantifying the results, and interpreting the outcomes.


    MtoZ Biolabs delivers reliable, rapid, and cost-effective MFA services, leveraging high stability, repeatability, and sensitivity systems for separation, characterization, identification, and quantification of MFA.

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