Mass Cytometry Sample Preparation and Analysis Steps

Sample Preparation

In mass spectrometry flow analysis, sample preparation is the first step of the journey and a crucial step that affects subsequent analysis results. The following steps are indispensable parts of the experimental process.

 

1. Sample Selection

When selecting samples, we need to consider the type and source of the sample, as well as the biological questions we want to investigate. Researchers can choose from a variety of samples, such as cells, tissues, blood, etc., and try to select samples that are homogeneous and in good condition.

 

2. Sample Processing

Sample processing includes cleaning, centrifugation, lysing, etc. We need to remove as many impurities as possible from the sample to ensure its purity for accurate analysis later.

 

Mass Spectrometry Flow Analysis Steps

After completing the sample preparation, we will enter the mass spectrometry flow analysis stage. This includes unique steps such as sample injection and mass spectrometry analysis.

 

1. Sample Injection

The researcher injects the processed sample into the mass spectrometry flow device. The device will push the sample forward at a constant speed to ensure that every component in the sample can be measured.

 

2. Mass Spectrometry Analysis

During the analysis, the device will separate and measure the incoming sample. In this step, we will obtain the mass information of each component, which will help us understand the structure and composition of the sample.

 

Data Parsing and Reproduction

Finally, through a series of complex but indispensable data processing, we will clearly present the measurement results for researchers to further in-depth analysis.