MALDI Microbial Identification Service
- Clinical microbiology and pathogen detection
- Food safety and quality control
- Pharmaceutical production and contamination monitoring
- Environmental microbiology and biodiversity studies
- Research on microbial taxonomy and phylogeny
- Validation of microbial strains in industrial fermentation
- Water quality testing and pathogen surveillance
- Disease outbreak investigation and epidemiology
The MALDI Microbial Identification Service utilizes Matrix-Assisted Laser Desorption/Ionization Time-of-Flight (MALDI-TOF) mass spectrometry, a cutting-edge technique for identifying microorganisms by analyzing their unique protein mass spectral fingerprints. MtoZ Biolabs offers high-quality MALDI Microbial Identification Service by leveraging advanced MALDI-TOF systems and expertly curated databases. MtoZ Biolabs' MALDI Microbial Identification Service ensures fast and accurate identification of a wide range of microbial such as bacteria, fungi, and yeasts.
Microbial identification is critical in fields such as clinical diagnostics, food safety, pharmaceutical quality control, and environmental monitoring. Traditional methods, including culturing, biochemical assays, and genetic sequencing, are often time-consuming, labor-intensive, and limited in their ability to analyze diverse samples efficiently. The MALDI microbial identification approach surpasses these methods with its unparalleled speed, cost-effectiveness, and high-throughput capabilities.
The MALDI microbial identification method is based on the principle of protein mass fingerprinting, leveraging the unique protein profiles of microorganisms. Every microorganism produces a distinct set of ribosomal and cellular proteins, which act as molecular fingerprints. These proteins are highly abundant, conserved within species, and distinct between species, making them ideal for identification purposes. In the process, microbial proteins are extracted and ionized, generating a mass spectrum that represents the protein composition of the microorganism. The resulting spectral profile is compared against a comprehensive reference database containing pre-analyzed spectra of known microbial species. This allows for accurate and rapid species-level identification by matching the spectral "fingerprint" to its closest reference in the database.
Figure 1. Workflow for MALDI Microbial Identification Service
Service Advantages
1. Rapid and Efficient
The MALDI Microbial Identification Service delivers species-level results within minutes, significantly reducing analysis time compared to traditional methods.
2. Advanced Platform
Leveraging cutting-edge MALDI Microbial Identification Service platform and expert analysis, MtoZ Biolabs ensures high sensitivity, reproducibility, and precision in microbial identification.
3. Comprehensive Database
Equipped with an extensive, curated reference database, the MALDI Microbial Identification Service ensures accurate identification of a wide range of microbial such as bacteria, fungi, and yeasts.
4. One-Stop Solution
From sample preparation to data analysis, the MALDI Microbial Identification Service offers a seamless workflow, saving your time and efforts.
5. High-Data-Quality
Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrates all MALDI Microbial Identification Service data, providing clients with a comprehensive data report.
Applications
Our MALDI Microbial Identification Service is applicable in various sectors including:
Case Study
High-Specificity Detection of E. coli in Fish Samples Using MALDI Microbial Identification Service
In this study, E. coli served as a model bacterium to evaluate the effectiveness of a novel protocol combining anti-E. coli magnetic beads with MALDI-TOF analysis. Magnetic beads successfully captured E. coli from fish sarcoplasmic protein (FSP) and muscle protein extracts with high reproducibility and sensitivity. A partial least-squares discriminant analysis (PLS-DA) plot showed clear discrimination of E. coli in matrices of varying complexity, including water, fish muscle surface, and protein extracts. The method achieved excellent accuracy (RSD = 3%) and identified 10 biomarker ions specific to E. coli, even in the presence of interfering bacteria. This study highlights the potential of the MALDI Microbial Identification Service as a reliable tool for enhancing food safety and ensuring the accurate detection of microbial contaminants in complex food matrices.
Chai, Z. S. et al. Food Chem X. 2022.
FAQ
Q: What are the main advantages of MALDI microbial identification compared to gene sequencing?
MALDI microbial identification is faster, more cost-effective, and requires less complex sample preparation compared to gene sequencing. It provides real-time species-level identification using conserved ribosomal protein fingerprints, while gene sequencing involves longer workflows and higher costs.
Deliverables
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Data Analysis, Preprocessing, and Estimation
4. The Detailed Information of Microbial Identification
5. Bioinformatics Analysis
6. Raw Data Files
Tailored for diverse applications, MtoZ Biolabs' MALDI Microbial Identification Service ensures precision and efficiency. Contact us today to discover how we can support your microbial research and analytical needs.
How to order?
