LC-MS Proteomics Service
- Cell Samples: Complete cell lysates or properly processed cell suspensions. Prompt sample handling is advised to prevent protein degradation.
- Tissue Samples: Rapid freezing or liquid nitrogen preservation is recommended to protect protein integrity.
- Blood and Serum Samples: Should be freshly collected or stored at low temperatures to prevent protein degradation. Use of anticoagulants is recommended to avoid clotting.
- Other Body Fluids: For fluids such as urine and saliva, follow standard collection and preservation procedures to ensure sample stability.
LC-MS proteomics is an advanced analytical approach that combines liquid chromatography with high-resolution mass spectrometry to provide comprehensive analysis of proteins within complex biological samples. LC-MS proteomics enables accurate identification and quantification of proteins, addressing the complex protein network analysis challenges that traditional techniques struggle to resolve. It has broad applications in fundamental research, disease mechanism studies, drug discovery, clinical diagnostics, and more.
MtoZ Biolabs offers highly efficient and reliable LC-MS Proteomics Service, utilizing an advanced proteomics platform equipped with Thermo Fisher's Q Exactive HF and Orbitrap Fusion Lumos mass spectrometry, along with a Nano-LC chromatography system. These state-of-the-art instruments significantly enhance the sensitivity and accuracy of analyses, enabling the detection of low-abundance proteins in complex biological samples and supporting researchers in making breakthrough advancements across various fields.
Technical Principles
LC-MS proteomics involves separating proteins or peptides from biological samples through liquid chromatography, followed by their detection and analysis using mass spectrometry. The liquid chromatography component separates proteins based on their chemical properties, polarity, and molecular weight, simplifying complex samples into individual, analyzable components. The mass spectrometry component measures the mass-to-charge ratio (m/z) of peptide fragments, using database matching to enable precise protein identification. The use of high-resolution mass spectrometers significantly boosts the sensitivity and accuracy of LC-MS analyses in complex samples, establishing it as a cornerstone of proteomics research.
Analysis Workflow
To ensure high-quality outcomes from the LC-MS proteomics service, MtoZ Biolabs strictly adheres to a meticulously designed and optimized workflow, covering all stages from sample preparation to final data analysis. Each step is carefully controlled to guarantee accuracy and reproducibility, providing reliable proteomics data support to clients.
Service Advantages
1. High Sensitivity and Resolution
LC-MS proteomics service provides exceptional sensitivity and resolution, enabling the detection of low-abundance proteins in complex samples and delivering precise protein information.
2. High Throughput Capability
This service is ideal for the concurrent analysis of large sample sets, significantly improving research efficiency and fulfilling the needs of high-throughput proteomics studies.
3. Accurate Quantification
In addition to protein identification, LC-MS proteomics service enables precise quantification, making it suitable for comparative analysis of protein expression under various conditions.
4. Broad Application Range
Capable of handling a wide array of biological samples, including cells, tissues, and blood, the service is applicable across a variety of research areas, including basic science, disease mechanism analysis, and drug target discovery.
5. High-Data-Quality
Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrate all proteomics analysis data, providing clients with a comprehensive data report.
6. One-Time-Charge
Our pricing is transparent, no hidden fees or additional costs.
Sample Submission Suggestions
To ensure reliable LC-MS proteomics results, we recommend that clients provide high-quality samples, as the quality and handling of these samples directly impact analysis accuracy and data reliability. For any technical questions or support, please contact the MtoZ Biolabs team:
Case Study
Case 1: Application of LC-MS Proteomics in Host Bacteria Proteome
Using LC-MS proteomics, the study comprehensively analyzed the proteome of the emerging host bacterium Halomonas bluephagenesis, identifying numerous key proteins involved in core metabolism, environmental adaptability, and industrial production. The findings provided critical insights into the molecular mechanisms of environmental stress responses and biomanufacturing optimization, supporting the development of Halomonas bluephagenesis as a high-efficiency biomanufacturing host.
Russell, M. et al. Sci. Data. 2022.
Case 2: Application of LC-MS Proteomics in Immune System Research
LC-MS proteomics was applied to detect milk protein-derived peptides in infant thymus tissue, revealing dietary antigens' presence and their role in stimulating IgG-secreting plasma cell activation. This study offered key data linking dietary antigens to early immune system development, underscoring the crucial role of LC-MS proteomic in analyzing complex samples.
Cordero, H. et al. J. Allergy Clin. Immunol. 2023.
What Could be Included in the Report?
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment
4. Data Analysis, Preprocessing, and Estimation
5. Bioinformatics Analysis
6. Raw Data Files
MtoZ Biolabs’ LC-MS proteomics service supports a broad range of research fields, from basic science to clinical applications. Whether facilitating fundamental research or enabling clinical advancements, our service meets diverse needs and accelerates scientific and medical breakthroughs. Through detailed protein structural analysis, disease mechanism exploration, and drug discovery, MtoZ Biolabs is committed to supporting your research with reliable technical and data assurance. Free project evaluation, welcome to learn more details!
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