iTRAQ Analysis Service

In today's rapidly advancing fields of life sciences and medicine, precisely understanding the dynamic changes of biomolecules and their intrinsic mechanisms has become the key to scientific breakthroughs. Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) technology emerges as a powerful tool, akin to a magical key, opening the door for researchers to explore deeper and providing an exceptionally robust analytical approach.

 

As a cutting-edge quantitative proteomics service based on mass spectrometry, iTRAQ analysis service operates on a sophisticated core principle. In the initial sample preparation phase, researchers first enzymatically digest proteins from different experimental groups into peptide fragments. These peptides are then labeled using specialized reagents containing specific isotopic tags. Notably, while these isotopic tags share identical chemical structures, they have slight mass differences. They bind specifically and precisely to the amino terminus or lysine residues of the peptides. After labeling, the mixed peptides undergo mass spectrometry analysis for further data processing.

 

The principle of iTRAQ analysis service is primarily based on mass differences between isotopic tags. When differentially labeled peptides enter the mass spectrometer, they produce distinct peak clusters with specific mass shifts in the spectrum. By comparing the intensity of these peaks, researchers can achieve a deep quantitative analysis of relative protein levels, accurately measuring subtle differences in protein expression between samples. Moreover, when internal standards or known concentration reference standards are included, the captured mass spectrometry signals can be used to precisely determine absolute protein quantities, enabling absolute quantification.

 

Services at MtoZ Biolabs

Utilizing Thermo Fisher’s Q Exactive HF mass spectrometry platform and the Orbitrap Fusion Lumos mass spectrometry platform combined with Nano-LC, the comprehensive quantitative proteomics package based on iTRAQ analysis service offered by MtoZ Biolabs. Simply provide us with your experimental objectives and send us your protein samples—we will handle all subsequent processes, including protein digestion, peptide labeling, peptide separation, mass spectrometry analysis, raw data interpretation, and bioinformatics analysis. Additionally, MtoZ Biolabs provides quantitative proteomics services based on TMT/MultiNotch MS, catering to the diverse needs of our clients.

 



Figure 1. Flow Chart of Quantitative Proteomics Analysis.

 

The iTRAQ analysis service has the following features:

1. High Throughput: Capable of simultaneous separation and analysis of up to 8–10 samples in a single experiment.

 

2. Broad Applicability: Suitable for various biological samples, including animal, plant, microbial, cellular, and bodily fluid samples.

 

3. Excellent Reproducibility and Accurate Quantification: The separation and identification conditions remain consistent across all samples, ensuring high experimental reproducibility and enhanced quantification accuracy.

 

4. High Resolution: iTRAQ analysis service can be combined with high-resolution LC-MS/MS technology, allowing for accurate quantification and characterization of low-abundance proteins.

 

5. Comprehensive Data Output: Provides both qualitative and quantitative information of detected proteins simultaneously.

 

6. High Degree of Automation: Based on high-resolution liquid chromatography-mass spectrometry (LC-MS), enabling automated operations and rapid analysis.

 

Service Advantages

1. Advanced Analytical Platform

MtoZ Biolabs has established a state-of-the-art iTRAQ analysis service platform, ensuring reliable, fast, and highly accurate analytical services.

 

2. Transparent Pricing

Our pricing is transparent, with no hidden or additional fees.

 

3. High-Quality Data

Comprehensive data coverage with strict quality control. The AI-driven bioinformatics platform integrates all spatial proteomics data, providing customers with extensive and detailed analytical reports.

 

4. Customized Research Solutions

MtoZ Biolabs offers tailored services to address unique research challenges and specific experimental requirements.

 

Applications

1. Disease Research

iTRAQ technology can be applied to study changes in protein expression during disease progression and to identify disease-related biomarkers.

 

2. Drug Development

iTRAQ analysis service aids in studying drug mechanisms of action and discovering potential drug targets.

 

3. Agricultural Research

By analyzing protein expression changes in plants under different environmental conditions, iTRAQ technology can help improve crop yield and stress resistance.

 

4. Fundamental Biological Research

iTRAQ analysis service is instrumental in studying biological processes such as cellular signaling pathways and protein-protein interactions.

 

Case Study

Researchers utilized iTRAQ technology to label, separate, and precisely analyze serum proteins from patients with non-traumatic femoral head necrosis and healthy individuals. This approach enabled the accurate identification of differentially expressed proteins between the two groups. In one study, 137 statistically significant differentially expressed proteins were identified, including apolipoproteins, which were found to be closely associated with the onset of the disease. These differential proteins serve as potential biomarkers for early disease screening, allowing for timely diagnosis and improved treatment outcomes. Furthermore, by continuously monitoring changes in serum protein levels during patient treatment using iTRAQ technology, researchers can assess the effectiveness of therapeutic interventions in real-time. Physicians can adjust treatment strategies based on dynamic protein expression patterns, enabling personalized and precision medicine approaches for patients with non-traumatic femoral head necrosis. This advancement holds promise for improving patient prognosis, alleviating suffering, and enhancing overall quality of life.

 



Figure 2. iTRAQ Experimental Process.

 

MtoZ Biolabs, an integrated Chromatography and Mass Spectrometry (MS) Services Provider, provides advanced proteomics, metabolomics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample consumption. Free project evaluation, welcome to learn more details!