Integrative 16S-Metabolomics Analysis

16S rRNA gene sequencing is a fundamental tool for investigating microbial community structures. By analyzing microbial 16S rRNA genes, researchers can uncover the diversity and abundance of microbial populations. Metabolomics, on the other hand, focuses on the qualitative and quantitative analysis of metabolites, offering a comprehensive view of metabolic processes. The combination of 16S rRNA sequencing with metabolomics allows for a multidimensional approach to exploring the interactions between microbial communities and host metabolism.

 

MtoZ Biolabs offers a 16S-Metabolomics Analysis service, integrating high-throughput 16S rRNA gene sequencing with metabolomics (both untargeted and targeted approaches). The service first identifies microbial species and metabolites that show significant differences between sample groups. Next, correlation analyses between microbial and metabolite abundance levels reveal key relationships, helping to identify critical microbial species and metabolites that impact metabolic states. This integrated analysis provides innovative insights into how microbes influence host metabolism.

 

Analysis Workflow

 



Xian, M. H. et al. J Ethnopharmacol. 2022.

 

Service Advantages

Why Choose MtoZ Biolabs?

1. Comprehensive Analysis: The joint approach combines 16S rRNA sequencing with metabolomics to explore microbial diversity and functional metabolic relationships.

2. Innovative Data Integration: Correlating microbial species and metabolite abundance uncovers functional associations, revealing key species and metabolites.

3.High Sensitivity and Throughput: Advanced platforms enable the analysis of large sample volumes, detecting even low-abundance microbial species and metabolites.

4. Multi-dimensional Insights: Systems biology methods ensure the integration of multi-level data, leading to comprehensive biological insights.

 

Sample Submission Requirements

1. Sample Types

Suitable for feces, blood, tissues, soil, and other sample types.

 

2. Sample Volume

Provide sufficient DNA for 16S sequencing and at least 200 μL of liquid sample or 200 mg of solid sample for metabolomics analysis.

 

3. Sample Handling

Samples should be frozen immediately and avoid repeated freeze-thaw cycles to maintain stability.

 

Note: Provide details on sample collection and handling.

 

Applications

1. Agriculture and Forestry: Investigating mechanisms of stress resistance, growth and development processes, and breeding conservation studies.

2. Animal Husbandry: Studying meat and dairy quality and exploring pathogenic mechanisms.

3. Basic Medicine and Clinical Diagnosis: Identifying biomarkers, elucidating disease mechanisms, classifying diseases, and facilitating personalized treatments.

4. Biopharmaceuticals: Understanding mechanisms of drug action, conducting efficacy evaluations, and advancing drug development.

5. Microbial Research: Examining pathogenic mechanisms, resistance strategies, and pathogen-host interactions.

 

Deliverables

What Could be Included in the Report?

1. Comprehensive Experimental details

2. Materials, Instruments, and Methods

3. Total Ion Chromatogram & Quality Control Assessment (project-dependent)

4. Data Analysis, Preprocessing, and Estimation (project-dependent)

5. Bioinformatics Analysis

6. Raw Data Files

 

MtoZ Biolabs' 16S-Metabolomics Analysis Service combines cutting-edge sequencing and metabolomics platforms with multi-dimensional data integration, providing comprehensive and accurate support for research in microbial ecology, disease mechanisms, and metabolic regulation. Free project evaluation, welcome to learn more details! Our technical specialists are available to provide a free business assessment.