Glycosphingolipids Analysis Service

Glycosphingolipids are widely present in cell membranes and serum. They either exist in free form or are complexed with other proteins. The glycan head group determines the biological function of glycosphingolipids. Abnormal glycosylation of glycosphingolipids is closely related to various cancers, such as breast cancer, lung cancer, and brain tumors. Glycosphingolipid glycans are also involved in many cellular processes, such as signal transduction, receptor function, and cell differentiation. Therefore, detailed analysis of the glycan head group structure of glycosphingolipids is of great value for disease research.

 

Analysis Workflow

Comprehensive analysis of glycosphingolipid glycans requires the use of glycosidase, which has broad substrate specificity and supports high-throughput analysis. Glycosphingolipid analysis involves the following steps:

1. Extraction and Purification of Glycosphingolipids from Cell Membranes or Serum

2. Release of Glycosphingolipid Glycans using Glycosidase

3. Permethylation of Glycans

4. UPLC-HILIC-FLD Analysis Based on 2AB Labeling

5. Analysis of Permethylated Glycans by TOF-MS

If necessary, determination of glycan structure by MS/MS.