Glycolithocholic Acid Analysis Service

Glycolithocholic acid (GLCA), also known as lithocholylglycine, is an endogenous metabolite and a glycine-conjugated secondary bile acid. It is primarily formed by the conjugation of lithocholic acid with glycine, playing a vital role in bile acid metabolism and gut microbiota interactions. Structurally, GLCA is characterized by a 3alpha-hydroxy group, which sets it apart from other bile acids such as glycodeoxycholic acid and glycochenodeoxycholic acid. Synthesized in the liver, GLCA is conjugated with glycine and then secreted into bile, where it undergoes further metabolism by bacterial enzymes in the intestine. Notably, GLCA is also utilized in the diagnosis of ulcerative colitis (UC), nonalcoholic steatohepatitis (NASH), and primary sclerosing cholangitis (PSC). These distinct features make GLCA particularly significant in studies of bile acid metabolism, liver health, and gut microbiota dynamics.

 

Figure 1. Molecular Structure of Glycolithocholic Acid

 

MtoZ Biolabs offers Glycolithocholic Acid Analysis Service using high performance liquid chromatography-mass spectrometry (HPLC-MS). This advanced method combines the separation capabilities of HPLC with the detection power of MS, ensuring high sensitivity and specificity in GLCA detection.

 

Analysis Workflow

 



 

Service Advantages

1. High Sensitivity and Precision

HPLC-MS/MS provides highly sensitive and accurate measurements, suitable for detecting low concentrations of GLCA.

 

2. Rapid and Efficient

Optimized sample preparation and fast analysis ensure quick and efficient processing.

 

3. Wide Applicability

Suitable for various biological samples, including blood, urine, feces, and tissues.

 

4. Accurate and Reliable Data

Ensures high reproducibility and low error rates, supporting both research and clinical applications, providing reliable and specific results.

 

Sample Submission Requirements

Make sure the sample volume is greater than 0.2 g or 0.2 mL. For detailed sample submission information, please contact our customer service.

 

Deliverables

1. Experimental Procedures

2. Relevant Liquid Chromatography and Mass Spectrometry Parameters

3. Detailed Information on Glycolithocholic Acid

4. Raw Data

5. Custom Analysis Report

 

MtoZ Biolabs is dedicated to advancing your research and clinical projects by providing detailed insights into GLCA's extensive physiological roles through top-tier analytical techniques and expert services.