Fourier-Transform Infrared Analysis of Antibody Drugs

Antibody drugs are a type of drugs that treat diseases through artificially synthesized antibodies, which achieve the therapeutic purpose by specifically binding with the target molecule. Common types of antibody drugs include monoclonal antibodies, artificially synthesized antibody fragments, immunotoxins, antibody-drug conjugates, etc. Antibody drugs have shown significant efficacy in the treatment of various diseases, such as cancer, autoimmune diseases, inflammatory diseases, immune regulation, and ophthalmic diseases. Antibody drugs require us to deeply understand the biochemical properties and 3D structure of proteins, as the activity, stability, toxicity, efficacy, and shelf life of antibody drugs are all affected by the structure-activity relationship.

 

Fourier transform infrared spectroscopy (FT-IR) is a commonly used spectroscopic analysis technique, often used to study the molecular structure and chemical composition of antibody drugs. It is based on the principle of interaction between molecules and infrared radiation, and obtains information by measuring the intensity of infrared light absorbed or scattered by the sample.

 

For antibody drugs, FT-IR analysis can help researchers and producers understand the secondary structure of antibodies, such as α-helices, β-folds, and irregular curls. By analyzing the infrared spectrum of antibody drugs, possible folding abnormalities or denaturation can be detected in time to ensure the quality and efficacy of the drug.

 

MtoZ Biolabs has established an FT-IR analysis platform using Thermo's Nicolet series of instruments, offering you one-stop antibody drug FT-IR analysis services. We can measure the infrared spectrum of proteins and peptides in the sample and perform subsequent baseline correction, Gaussian deconvolution, second-order derivative fitting, and finally determine the secondary structure information of proteins and peptides in the sample based on the peak area. Contact us for a free project consultation.