Dynamic Light Scattering Analyzer Testing Steps

The Dynamic Light Scattering (DLS) analyzer is a device used for measuring the average dynamic diameter of particles such as nanoparticles, proteins, polymers, etc. The test process usually consists of the following basic stages:

 

Analysis Workflow

1. Sample Preparation

(1) Clean all sample bottles and utensils to avoid contamination.

(2) Prepare the sample solution, ensuring that the sample is within the appropriate concentration range to avoid multi-body effects or too low signal strength.

(3) Filter the sample to remove large particles or impurities, typically using a 0.22 micron or smaller pore size filter.

 

2. Device Calibration

Calibrate the DLS instrument according to the requirements of the instrument; it may be necessary to use specific standard particles for calibration.

 

3. Load Sample

Load the processed sample into the sample pool or sample slot of the DLS analyzer.

 

4. Set Parameters

Set appropriate test parameters in the software of the instrument, such as measurement time, temperature, and angle, etc.

 

5. Start Measurement

Start the measurement program, the instrument will record the changes in the scattering light intensity of the particles in the sample over time.

 

6. Data Analysis

(1) After the measurement is complete, the software will calculate the size distribution of the particles based on the scattering light data.

(2) Analyze the data to determine the average particle size and particle size distribution of the sample.

 

7. Cleaning and Maintenance

After the test is completed, clean the sample chamber and any parts that have come into contact with the sample to prevent cross-contamination.

 

Each step needs to be carefully executed to ensure the accuracy and repeatability of the test results. Different DLS instruments may have slightly different operation steps, so read and follow the user manual provided by the manufacturer carefully before operation.