Droplet-Based Single Cell Sequencing Service
- Cell Type: Suitable for a wide range of cell types, including tumor cells, immune cells, and stem cells.
- Cell Concentration: A sufficient number of viable cells is required, with a concentration of several million cells per milliliter.
- Sample Purity: Cells must be properly separated and purified to ensure reliable sequencing results.
- Cancer Research: Unveiling tumor cell heterogeneity, studying subtypes, and their resistance to therapies.
- Immunology: Analyzing immune cell function, subgroup differentiation, and dynamic changes during immune responses.
- Neuroscience: Investigating cell interactions within the nervous system and differences in neuronal functions.
- Stem Cell and Developmental Biology: Exploring gene expression during stem cell differentiation and understanding their pluripotency and differentiation potential.
- Disease Mechanism Research: Investigating the molecular mechanisms underlying diseases such as cardiovascular and metabolic disorders at the single-cell level.
Droplet-based single cell sequencing is a powerful genomic analysis approach that leverages microfluidic technology. By encapsulating individual cells in microdroplets, droplet-based single cell sequencing ensures that each cell can independently interact with reagents in a separate reaction chamber. This enables high-throughput, high-precision analysis at the single-cell level, overcoming the limitations of traditional methods in studying cellular heterogeneity and providing detailed gene expression profiles for each individual cell.
Droplet-based single cell sequencing is widely used across various fields, including cancer research, immunology, neuroscience, and stem cell research. It enables researchers to uncover individual differences and functional characteristics within cell populations. MtoZ Biolabs offers high-quality Droplet-Based Single Cell Sequencing Service leveraging its cutting-edge technological platform to help clients explore single-cell level data and providing robust data support for their research projects.
Technical Principles
Droplet-based single cell sequencing utilizes a microfluidic system to mix cells with reagents and encapsulate them in microdroplets, facilitating efficient genomic analysis. Initially, cells are precisely separated on a microfluidic chip and directed into droplets, with each droplet containing only one cell and the necessary reagents. Since each droplet functions as an independent reaction chamber, gene expression analysis occurs separately for each cell, minimizing the risk of cross-contamination between cells.
Inside the droplet, cells undergo RNA reverse transcription, where mRNA is transcribed into cDNA. Each droplet carries a unique barcode, which covalently binds to the cDNA fragments, linking them to their respective cells for subsequent analysis. Amplification of these cDNA fragments further enhances the detection sensitivity of low-abundance RNA.
The amplified cDNA fragments are then analyzed using high-throughput sequencing platforms such as Illumina. The high throughput of these platforms allows for the simultaneous analysis of thousands to tens of thousands of single-cell transcriptomes in a single experiment. Post-sequencing, advanced bioinformatics tools and algorithms enable comprehensive analysis of the single-cell data, uncovering cellular heterogeneity, cellular traits, and biological process differences between cells.
Zeng, Z. W. et al. Stem Cell Res. Ther. 2018.
Services at MtoZ Biolabs
MtoZ Biolabs offers Droplet-Based Single Cell Sequencing Service using the 10x Genomics Chromium system and the Illumina platform. Our services encompass everything from sample preparation to data analysis, ensuring the acquisition of high-quality single-cell gene expression data.
1. Sample Preparation and Cell Separation
We provide cell separation, purification, and viability testing to ensure that high-quality viable cells are available for sequencing.
2. Droplet Encapsulation and RNA Reverse Transcription
Using the 10x Genomics Chromium system, we precisely encapsulate individual cells in microdroplets, performing RNA reverse transcription and amplification to ensure accurate gene expression data for each cell.
3. High-Throughput Sequencing and Data Acquisition
Using the Illumina platform, we conduct high-throughput RNA sequencing to capture the transcriptomic data of each cell, ensuring both high-quality and high-sensitivity results.
4. Data Analysis and Interpretation
We offer comprehensive data analysis services, including cell clustering, gene expression profiling, and interpretation of cellular heterogeneity, enabling clients to derive actionable insights from their research.
Zeng, Z. W. et al. Stem Cell Res. Ther. 2018.
Why Choose MtoZ Biolabs?
1. High Throughput and Precision: Droplet-Based Single Cell Sequencing Service allows for the simultaneous analysis of thousands to tens of thousands of single cells, providing high-resolution gene expression data to reveal subtle intercellular differences.
2. Sensitivity and Low-Abundance Detection: It is capable of detecting low-abundance genes, making it ideal for studying rare cell types or faint biological signals.
3. Single-Cell Resolution: Each cell's gene expression can be independently analyzed, providing accurate information on cellular heterogeneity and function.
4. Database Independence: Droplet-Based Single Cell Sequencing Service does not rely on pre-existing databases, enabling independent data analysis and offering more biological insights.
5. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
Sample Submission Suggestions
To ensure optimal droplet-based single cell sequencing results, we recommend the following for sample preparation:
MtoZ Biolabs provides detailed sample submission guidelines to ensure compliance with our standards throughout the sequencing process. Please contact us for any additional assistance.
Applications
Our Droplet-Based Single Cell Sequencing Service is applicable in the following research fields:
Case Study
Case 1: Combining droplet-based single cell sequencing with long-read technology, the diversity of single-cell transcript isoforms was revealed.
Tian, L. Y. et al. Genome Biol. 2021.
MtoZ Biolabs offers advanced, accurate, and efficient Droplet-Based Single Cell Sequencing Service. Whether you are conducting basic research, disease studies, or drug development, we provide high-quality data to support your investigations into cellular-level biological phenomena. Please reach out to us for customized solutions tailored to your research needs.
How to order?
